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Titolo:
EXPRESSION OF SMALL EXTRACELLULAR CHONDROITIN DERMATAN SULFATE PROTEOGLYCANS IS DIFFERENTIALLY REGULATED IN HUMAN ENDOTHELIAL-CELLS/
Autore:
NELIMARKKA L; KAINULAINEN V; SCHONHERR E; MOISANDER S; JORTIKKA M; LAMMI M; ELENIUS K; JALKANEN M; JARVELAINEN H;
Indirizzi:
UNIV TURKU,DEPT MED BIOCHEM,KIINAMYLLYNKATU 10 FIN-20520 TURKU FINLAND UNIV TURKU,DEPT MED BIOCHEM FIN-20520 TURKU FINLAND UNIV TURKU,DEPT INTERNAL MED FIN-20520 TURKU FINLAND TURKU CTR BIOTECHNOL FIN-20520 TURKU FINLAND UNIV MUNSTER,INST PHYSIOL CHEM & PATHOBIOCHEM GER-4400 MUNSTER GERMANY UNIV KUOPIO,DEPT ANAT FIN-70211 KUOPIO FINLAND
Titolo Testata:
The Journal of biological chemistry
fascicolo: 19, volume: 272, anno: 1997,
pagine: 12730 - 12737
SICI:
0021-9258(1997)272:19<12730:EOSECD>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; BOVINE ARTICULAR-CARTILAGE; MAMMARY EPITHELIAL-CELLS; DECORIN GENE-EXPRESSION; NECROSIS-FACTOR-ALPHA; GROWTH-FACTOR-BETA; DERMATAN SULFATE; CORE PROTEIN; SURFACE PROTEOGLYCANS; OSTEOINDUCTIVE FACTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
82
Recensione:
Indirizzi per estratti:
Citazione:
L. Nelimarkka et al., "EXPRESSION OF SMALL EXTRACELLULAR CHONDROITIN DERMATAN SULFATE PROTEOGLYCANS IS DIFFERENTIALLY REGULATED IN HUMAN ENDOTHELIAL-CELLS/", The Journal of biological chemistry, 272(19), 1997, pp. 12730-12737

Abstract

We have examined the expression of the small extracellular chondroitin/dermatan sulfate proteoglycans (CS/DS PGs), biglycan, decorin, and PG-100, which is the proteoglycan form of colony stimulating factor-1, in the human endothelial cell line EA.hy 926. We have also examined whether modulation of the phenotype of EA.hy 926 cells by tumor necrosisfactor-alpha (TNF-alpha) is associated with specific changes in the synthesis of these PGs. We demonstrate that EA.hy 926 cells, when they form monolayer cultures typical of macrovascular endothelial cells, express and synthesize detectable amounts of biglycan and PG-100, but not decorin. On SDS-polyacrylamide gel electrophoresis both PGs behave like proteins of the relative molecular weight of similar to 250,000, TNF-alpha that changed the morphology of the cells from a polygonal shape into a spindle shape and that also stimulated the detachment of thecells from culture dish, markedly decreased the net synthesis of biglycan, whereas the net synthesis of PG-100 was increased. These changeswere parallel with those observed at the mRNA level of the corresponding PGs. The proportions of the different sulfated CS/DS disaccharide units of PGs were not affected by TNF-alpha. Several other growth factors/cytokines, such as interferon-gamma, fibroblast growth factors-2 (FGF-2) and -7 (FGF-7), interleukin-1 beta, and transforming growth factor-beta, unlike TNF-alpha, modulated neither the morphology nor the biglycan expression of EA.hy 926 cells under the conditions used in theexperiments. However, PG-100 expression was increased also in response to PGF-2 and -7 and transforming growth factor-beta. None of the above cytokines, including TNF-alpha, was able to induce decorin expression in the cells. Our results indicate that the regulatory elements controlling the expression of the small extracellular CS/DS PGs in human endothelial cells are different.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/08/20 alle ore 12:43:43