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Titolo:
GROUP-II PHOSPHOLIPASE-A2 IN HUMAN MALE REPRODUCTIVE-ORGANS AND GENITAL TUMORS
Autore:
KALLAJOKI M; ALANEN KA; NEVALAINEN M; NEVALAINEN TJ;
Indirizzi:
UNIV TURKU,DEPT PATHOL,KIINAMYLLYNKATU 10 FIN-20520 TURKU FINLAND TURKU UNIV HOSP FIN-20520 TURKU FINLAND TURKU UNIV,DEPT ANAT TURKU FINLAND
Titolo Testata:
The Prostate
fascicolo: 4, volume: 35, anno: 1998,
pagine: 263 - 272
SICI:
0270-4137(1998)35:4<263:GPIHMR>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SEMINAL PLASMA; EXPRESSION; PURIFICATION; CELLS; SEQUENCE; FLUID; ACID; A(2);
Keywords:
GROUP II PHOSPHOLIPASE A2; PROSTATE; PROSTATIC CANCER; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; NORTHERN BLOTTING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
M. Kallajoki et al., "GROUP-II PHOSPHOLIPASE-A2 IN HUMAN MALE REPRODUCTIVE-ORGANS AND GENITAL TUMORS", The Prostate, 35(4), 1998, pp. 263-272

Abstract

BACKGROUND. Group II phospholipase A2 (PLA2) is a lipolytic enzyme suggested to play a role in inflammation and antibacterial defence. In seminal fluid, the concentration of PLA2 is exceedingly high under normal circumstances (about 1,000 times the concentration in blood plasma of healthy humans). To elucidate the origin of the enzyme present in seminal plasma, we investigated the expression of group II PLA2 in malereproductive organs both at protein and mRNA levels. In addition, thepresence of the enzyme was studied in common male genital tumors. METHODS. The methods used were immunocytochemistry, in situ hybridization, and Northern blotting. RESULTS. Northern blotting gave positive results for group II PLA2 mRNA in normal prostate, whereas other normal genital tissues gave negative results. Immunohistochemistry and in situ hybridization of group II PLA2 gave identical results. The enzyme was produced exclusively by the secretory epithelial cells of the prostatic gland. Surprisingly, expression was restricted to the posterior lobeand paraurethral glands of the prostate. Cells of prostatic adenocarcinoma expressed group II PLA2, whereas cells of other male genital tumors contained neither the enzyme protein nor the mRNA of group II PLA2. In some cases prostatic cancer cell seemed to express group II PLA2 at a higher rate than normal prostatic gland cells. CONCLUSIONS. The high content of group II PLA2 in seminal plasma is due to the local production and secretion of the enzyme by the epithelial cells of the prostatic glands. Group II PLA2 is expressed focally, suggesting that specialized prostatic glands secrete this enzyme. All prostatic adenocarcinomas tested expressed group II PLA2 in variable amounts. (C) 1998 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 20:13:29