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Titolo:
TARGETED RADIOTHERAPY OF MULTICELL NEUROBLASTOMA SPHEROIDS WITH HIGH SPECIFIC ACTIVITY [I-125] METAIODOBENZYLGUANIDINE
Autore:
ROA WHY; MILLER GG; MCEWAN AJB; MCQUARRIE SA; TSE J; WU J; WIEBE LI;
Indirizzi:
CROSS CANC INST,DEPT ONCOL,11560 UNIV AVE EDMONTON AB T6G 1Z2 CANADA UNIV ALBERTA,FAC MED & ORAL HLTH SCI EDMONTON AB T6G 2M7 CANADA UNIV ALBERTA,DEPT ONCOL EDMONTON AB T6G 2M7 CANADA UNIV ALBERTA,DEPT RADIOL & DIAGNOST IMAGING EDMONTON AB T6G 2M7 CANADA UNIV ALBERTA,FAC PHARM & PHARMACEUT SCI EDMONTON AB T6G 2M7 CANADA
Titolo Testata:
International journal of radiation oncology, biology, physics
fascicolo: 2, volume: 41, anno: 1998,
pagine: 425 - 432
SICI:
0360-3016(1998)41:2<425:TROMNS>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
B-CELL LYMPHOMA; SK-N-SH; NEURO-BLASTOMA; MAMMALIAN-CELLS; MONOCLONAL-ANTIBODIES; LOW-LET; RADIOIMMUNOTHERAPY; RADIATION; I-125; DOSIMETRY;
Keywords:
I-125 MIBG; NEUROBLASTOMA; MULTICELL SPHEROIDS; FLOW CYTOMETRY; TARGETED RADIOTHERAPY; DOSIMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
W.H.Y. Roa et al., "TARGETED RADIOTHERAPY OF MULTICELL NEUROBLASTOMA SPHEROIDS WITH HIGH SPECIFIC ACTIVITY [I-125] METAIODOBENZYLGUANIDINE", International journal of radiation oncology, biology, physics, 41(2), 1998, pp. 425-432

Abstract

Purpose: Iodine-125 induces cell death by a mechanism similar to thatof high linear energy transfer (high-LET) radiation. This study investigates the cytotoxicity of high-specific-activity [I-125]meta-iodobenzylguanidine (I-125-mIBG) in human SK-N-MC neuroblastoma cells grown as three-dimensional multicellular spheroids. Materials and Methods: Spheroids were incubated with high-specific-activity I-125-mIBG (6 mCi/mu g, 1000 times that of the conventional specific activity used for autoradiography). Cytotoxicity was assessed by fluorescence viability markers and confocal microscopy for intact spheroids, fluorescence-activated cell sorting and clonogenic assay, and clonogenic assays for dispersed whole spheroids. Distribution of radioactive mIBG was determinedby quantitative light-microscope autoradiography of spheroid cryostatsections. Dose estimation was based on temporal knowledge of the retained radioactivity inside spheroids, and of the radiolabel's emission characteristics. Findings were compared with those of spheroids treated under the same conditions with I-131-mIBG, cold mIBG, and free iodine-125. Results: I-125-mIBG exerted significant cell killing. Complete spheroids were eradicated when they were treated with 500 mu Ci of I-125-mIBG, while those treated with 500 mu Ci or 1000 mu Ci of I-131-mIBG were not, The observed difference in cytotoxicity between treatmentswith I-125- and I-131-mIBG could not be accounted for by the absorbeddose of spheroid alone. The peripheral, proliferating cell layer of the spheroids remained viable at the moderate radioactivity of 100 mu Ci for both isotopes. Cytotoxicity induced by I-125-mIBG was quantitatively comparable by the peripheral rim thickness to that of I-131-mIBG at the dose of 100 mu Ci. The peripheral rim thickness decreased most significantly in the first 17 hours after initial treatment, There wasno statistical decrease in the rim thickness identified afterwards for the second, third, and fourth days of incubation. Conclusion: The cytotoxic effect of high-specific-activity I-125-mIBG appears to be comparable to, if not more efficient than that of conventionally used I-131-mIBG at the same level of total radioactivity. I-125-mIBG may improve the therapeutic index over that of I-131-mIBG in the clinical management of metastatic neuroblastoma due to the short range of Auger electrons. (C) 1998 Elsevier Science Inc.

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Documento generato il 25/01/20 alle ore 18:53:39