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Titolo:
HOMOLOGY-DEPENDENT GENE SILENCING IN PARAMECIUM
Autore:
RUIZ F; VAYSSIE L; KLOTZ C; SPERLING L; MADEDDU L;
Indirizzi:
CNRS,CTR GENET MOL F-91198 GIF SUR YVETTE FRANCE CNRS,CTR GENET MOL F-91198 GIF SUR YVETTE FRANCE
Titolo Testata:
Molecular biology of the cell
fascicolo: 4, volume: 9, anno: 1998,
pagine: 931 - 943
SICI:
1059-1524(1998)9:4<931:HGSIP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFRACILIARY LATTICE; SEQUENCE-ANALYSIS; DNA; TETRAURELIA; PROTEINS; MUTANTS; CENTRIN; TRANSFORMATION; MICROINJECTION; MORPHOGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
F. Ruiz et al., "HOMOLOGY-DEPENDENT GENE SILENCING IN PARAMECIUM", Molecular biology of the cell, 9(4), 1998, pp. 931-943

Abstract

Microinjection at high copy number of plasmids containing only the coding region of a gene into the Paramecium somatic macronucleus led to a marked reduction in the expression of the corresponding endogenous gene(s). The silencing effect, which is stably maintained throughout vegetative growth, has been observed for all Paramecium genes examined so far: a single-copy gene (ND7), as well as members of multigene families (centrin genes and trichocyst matrix protein genes) in which all closely related paralogous genes appeared to be affected. This phenomenon may be related to posttranscriptional gene silencing in transgenic plants and quelling in Neurospora and allows the efficient creation ofspecific mutant phenotypes thus providing a potentially powerful toolto study gene function in Paramecium. For the two multigene families that encode proteins that coassemble to build up complex subcellular structures the analysis presented herein provides the first experimental evidence that the members of these gene families are not functionally redundant.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 22:13:02