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Titolo:
GATA4 TRANSCRIPTION FACTOR IS REQUIRED FOR VENTRAL MORPHOGENESIS AND HEART TUBE FORMATION
Autore:
KUO CT; MORRISEY EE; ANANDAPPA R; SIGRIST K; LU MM; PARMACEK MS; SOUDAIS C; LEIDEN JM;
Indirizzi:
UNIV CHICAGO,DEPT MED,5841 S MARYLAND AVE CHICAGO IL 60637 UNIV CHICAGO,DEPT MED CHICAGO IL 60637 UNIV CHICAGO,DEPT PATHOL CHICAGO IL 60637 UNIV CHICAGO,COMM GENET CHICAGO IL 60637
Titolo Testata:
Genes & development
fascicolo: 8, volume: 11, anno: 1997,
pagine: 1048 - 1060
SICI:
0890-9369(1997)11:8<1048:GTFIRF>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
EMBRYONIC STEM-CELLS; HEAVY-CHAIN GENE; IN-VITRO; ENDOTHELIAL-CELLS; ANTERIOR ENDODERM; SKELETAL-MUSCLE; BINDING PROTEIN; RETINOIC ACID; MOUSE EMBRYOS; EXPRESSION;
Keywords:
GATA4; GENE TARGETING; PRECARDIAC MESODERM; VENTRAL MORPHOGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
C.T. Kuo et al., "GATA4 TRANSCRIPTION FACTOR IS REQUIRED FOR VENTRAL MORPHOGENESIS AND HEART TUBE FORMATION", Genes & development, 11(8), 1997, pp. 1048-1060

Abstract

Previous studies have suggested that the GATA4 transcription factor plays an important role in regulating mammalian cardiac development. Inthe studies described in this report we have used gene targeting to produce GATA4-deficient mice. Homozygous GATA4-deficient (GATA4(-/-)) mice died between 8.5 and 10.5 days post coitum (dpc). GATA4(-/-) embryos displayed severe defects in both rostral-to-caudal and lateral-to-ventral folding, which were reflected in a generalized disruption of the ventral body pattern. This resulted in the defective formation of anorganized foregut and anterior intestinal pore, the failure to close both the amniotic cavity and yolk sac, and the uniform lack of a ventral pericardial cavity and heart tube. Analysis of cardiac development in the GATA4(-/-) mice demonstrated that these embryos developed splanchnic mesoderm, which differentiated into primitive cardiac myocytes that expressed contractile proteins. However, consistent with the observed defect in ventral morphogenesis, these GATA4(-/-) procardiomyocytes failed to migrate to the ventral midline to form a linear heart tubeand instead formed aberrant cardiac structures in the anterior and dorsolateral regions of the embryo. The defect in ventral migration of the GATA4(-/-) procardiomyocytes was not cell intrinsic because GATA4(-/-) cardiac myocytes and endocardial cells populated the hearts of GATA4(-/-)-C57BL/6 chimeric mice. Taken together, these results demonstrated that GATA4 is not essential for the specification of the cardiac cell lineages. However, they define a critical role for GATA4 in regulating the rostral-to-caudal and lateral-to-ventral folding of the embryo that is needed for normal cardiac morphogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 15:14:01