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Titolo:
SANDFLY MIDGUT LECTIN - EFFECT OF GALACTOSAMINE ON LEISHMANIA-MAJOR INFECTIONS
Autore:
VOLF P; KIEWEGOVA A; SVOBODOVA M;
Indirizzi:
CHARLES UNIV,DEPT PARASITOL,VINICNA 7 CR-12844 PRAGUE 2 CZECH REPUBLIC
Titolo Testata:
Medical and veterinary entomology
fascicolo: 2, volume: 12, anno: 1998,
pagine: 151 - 154
SICI:
0269-283X(1998)12:2<151:SML-EO>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHLEBOTOMINE SANDFLIES; TRYPANOSOMA-BRUCEI; GUT; PROMASTIGOTES; AGGLUTINATION; GLOSSINA; EXTRACTS; TSETSE; FLIES;
Keywords:
LEISHMANIA MAJOR; PHLEBOTOMUS DUBOSCQI; SANDFLIES; LEISHMANIASIS VECTORS; VECTOR COMPETENCE; LECTIN INHIBITION; MIDGUT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
P. Volf et al., "SANDFLY MIDGUT LECTIN - EFFECT OF GALACTOSAMINE ON LEISHMANIA-MAJOR INFECTIONS", Medical and veterinary entomology, 12(2), 1998, pp. 151-154

Abstract

Galactosamine, which has been shown in vitro to specifically inhibit sandfly midgut lectin activity, was fed to Phlebotomus duboscqi females with blood containing promastigotes of Leishmania major. Non-inhibitory sugar, galactose, was added in controls, For two strains of L. major (LV 561 and Neal-P), galactosamine substantially enhanced the establishment of infection in the sandfly posterior midgut and significantly increased parasite loads after defaecation, but did not affect anterior migration of Leishmania. On day 3 post-infection, most infections in galactosamine-fed sandfly groups (92% of LV 561 and 100% of Neal-P)were found in the ectoperitrophic space of the posterior midgut, whereas most infections in the galactose-fed groups of sandflies (85% in LV 561 and 96% in Neal-P) were restricted to the peritrophic sac. On day 9, however, the proportion of infections colonizing the stomodeal valve was similar in both dietary groups of sandflies for both strains of L, major. The addition of galactosamine prevented the decrease of parasite loads which occurred in controls between days 3 and 6 post-infection. On days 6 and 9, heavy infections were observed almost exclusively in galactosamine-fed females. Differences between groups were morepronounced for the Neal-P strain, which normally developed poorly in sandflies. Morphology of L. major LV 561 was not affected by galactosamine supplement: the lengths of parasite body and flagellum were similar in both sandfly groups. Two hypotheses are considered for the role of sandfly midgut lectin in Leishmania development in the vector midgut. One proposes that sandfly lectin kills Leishmania promastigotes, the other assumes that lectin blocks LPG-mediated binding of promastigotes to sandfly midgut microvilli.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 23:08:55