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Titolo:
USE OF DYES AS INDIRECT DETECTION PROBES FOR THE HIGH-SENSITIVITY DETERMINATION OF ANIONS BY CAPILLARY ELECTROPHORESIS
Autore:
DOBLE P; MACKA M; HADDAD PR;
Indirizzi:
UNIV TASMANIA,DEPT CHEM,GPO BOX 252-75 HOBART TAS 7001 AUSTRALIA UNIV TASMANIA,DEPT CHEM HOBART TAS 7001 AUSTRALIA
Titolo Testata:
Journal of chromatography
fascicolo: 1-2, volume: 804, anno: 1998,
pagine: 327 - 336
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDIRECT PHOTOMETRIC DETECTION; INDIRECT UV DETECTION; ZONE ELECTROPHORESIS; CELL;
Keywords:
BUFFER COMPOSITIONS; DYES; BROMOCRESOL GREEN; INDIGO-TETRASULFONATE; ALKANESULFONIC ACIDS; INORGANIC ANIONS; ORGANIC ACIDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
P. Doble et al., "USE OF DYES AS INDIRECT DETECTION PROBES FOR THE HIGH-SENSITIVITY DETERMINATION OF ANIONS BY CAPILLARY ELECTROPHORESIS", Journal of chromatography, 804(1-2), 1998, pp. 327-336

Abstract

High sensitivity for indirect detection was achieved by utilising highly absorbing species as the displaced co-ion (or probe). Two highly absorbing dyes, bromocresol green and indigo-tetrasulfonate, were investigated as potential probes in the determination of small organic and inorganic anions. The concentration of these probes was kept as low aspossible to ensure the signal-to-noise ratios were reasonable and thebackground absorbance was within the linear range of the detector. Four different protocols for buffering the electrolyte with such low probe concentrations were investigated. Buffering with agents that introduce co-anions [acetate or 2-(cyclohexylamino)ethanesulfonic acid (CHES) buffers] proved unsuitable as detection sensitivities were diminished due to competitive displacement by the analytes and system peaks were also induced. Buffering without introduction of co-anions was achieved using the buffering base, diethanolamine, or the use of ampholytes,lysine and glutamic acid. For separations performed with these two buffering approaches, migration time reproducibilities were less than 1%R.S.D. for most analytes. Minimal detectable amounts were in the low attomol region (1.10(-18) mel), corresponding to sub-mu M vacuum injected solution concentrations. These were an order of magnitude lower than the general detection limit reported for indirect photometric detection, and were comparable with detection limits achieved with indirectfluorescence detection. Finally, the detection limits were further improved by approximately three times for anions analyser with indigo-tetrasulfonate as the probe when a Z cell was employed as the detection cell. (C) 1998 Elsevier Science B.V.

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Documento generato il 14/07/20 alle ore 09:20:13