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Titolo:
RAF-1 INDEPENDENT STIMULATION OF MITOGEN-ACTIVATED PROTEIN-KINASE BY LEUKEMIA INHIBITORY FACTOR IN 3T3-L1 CELLS
Autore:
SCHIEMANN WP; NATHANSON NM;
Indirizzi:
UNIV WASHINGTON,DEPT PHARMACOL SEATTLE WA 98195 UNIV WASHINGTON,DEPT PHARMACOL SEATTLE WA 98195
Titolo Testata:
Oncogene
fascicolo: 20, volume: 16, anno: 1998,
pagine: 2671 - 2679
SICI:
0950-9232(1998)16:20<2671:RISOMP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSDUCING RECEPTOR COMPONENT; SIGNAL-REGULATED KINASE-2; EPIDERMAL GROWTH-FACTOR; MAP KINASE; PC12 CELLS; SERINE PHOSPHORYLATION; C-RAF; TYROSINE PHOSPHORYLATION; HEMATOPOIETIC-CELLS; MYELOID-LEUKEMIA;
Keywords:
CYTOKINE; LEUKEMIA INHIBITORY FACTOR; MAP KINASE; SIGNAL TRANSDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
W.P. Schiemann e N.M. Nathanson, "RAF-1 INDEPENDENT STIMULATION OF MITOGEN-ACTIVATED PROTEIN-KINASE BY LEUKEMIA INHIBITORY FACTOR IN 3T3-L1 CELLS", Oncogene, 16(20), 1998, pp. 2671-2679

Abstract

We show here that treatment of 3T3-L1 cells with leukemia inhibitory factor (LIF) stimulated Raf-l activity in a time-and dose-dependent manner, Although phorbol ester failed to activate Raf-l directly, a protein kinase C-stimulated signal was found to be necessary, but not sufficient, for LIF-mediated activation of Raf-l, Elevation of intracellular cAMP levels completely blocked Raf-l activation by LIF, but was without effect on the magnitude of mitogen-activated protein kinase (MAPK) stimulation by the cytokine, suggesting the presence of a Raf-l-independent, cAMP-insensitive MAPK kinase kinase (MAPKKK) pathway in 3T3-L1 cells, Mono Q-fractionation of LIF-stimulated 3T3-L1 extracts identified a single peak of MAPKKK activity that was largely insensitive to elevated intracellular levels of cAMP, and that failed to correlate with stimulation of either Raf-l or MEKK1 protein kinases, Our results demonstrate that LIF-mediated activation of the MAP kinase cascade in 3T3-L1 cells proceeds through both Raf-l-dependent and -independent pathways which differ in their sensitivity to inhibition by intracellularcAMP.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 07:32:12