Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
B-CELL EPITOPES IN HIV-1 TAT AND REV PROTEINS COLOCALIZE WITH T-CELL EPITOPES AND WITH FUNCTIONAL DOMAINS
Autore:
TAHTINEN M; RANKI A; VALLE SL; OVOD V; KROHN K;
Indirizzi:
TAMPERE UNIV,INST MED TECHNOL FIN-33101 TAMPERE FINLAND TAMPERE UNIV HOSP TAMPERE FINLAND UNIV HELSINKI,DEPT DERMATOL & VENEREAL DIS HELSINKI FINLAND
Titolo Testata:
Biomedicine & pharmacotherapy
fascicolo: 10, volume: 51, anno: 1997,
pagine: 480 - 487
SICI:
0753-3322(1997)51:10<480:BEIHTA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; ANTIBODY-RESPONSE; MONOCLONAL-ANTIBODIES; NATURAL ANTIBODIES; SYNTHETIC PEPTIDES; GENE-EXPRESSION; INDIVIDUALS; AIDS; LOCALIZATION; PROGRESSION;
Keywords:
TAT; REV; B-CELL EPITOPES; SYNTHETIC PEPTIDES; EPITOPE MAPPING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
M. Tahtinen et al., "B-CELL EPITOPES IN HIV-1 TAT AND REV PROTEINS COLOCALIZE WITH T-CELL EPITOPES AND WITH FUNCTIONAL DOMAINS", Biomedicine & pharmacotherapy, 51(10), 1997, pp. 480-487

Abstract

We describe the characterization of the B-cell epitopes of HIV-1 regulatory proteins Tar and Rev. The prevalence of antibodies to these proteins among human immunodeficiency virus (HIV)-1-infected individuals was examined by enzyme-linked immunosorbent assay (ELISA) and by Western blotting. The Tat and Rev antibody-positive sera were selected for epitope mapping performed with partially overlapping synthetic peptides bound to polyethylene pins. Eighteen and twelve percent of HIV-infected individuals had antibodies against Tar or Rev, respectively. In Tar, four epitopic regions were identified, situated within amino acids 6-10 (PRLEP), 21-37 (ACTNCYCKKCCFHCQVC), 39-58 (ITKALGISYGRKKRRQRRRA) and 74-82 (TSQSRGDPT). The most frequently recognized epitopic regionswere located in the middle of the protein. In Rev, the two most frequently recognized epitopic regions were near the amino terminus of the protein within amino acids 12-20 (LIRTVRLIK) and 38-49 (RRNRRRRWRERQ). A third epitope was mapped around amino acids 55-62 (ISERILGT) and a fourth around amino acids 78-83 (LERLTL). To analyze the specificity of Tat and Rev epitopes, soluble synthetic peptides representing the identified epitopes were used in an ELISA assay, and the recognition of most epitopes was shown to be specific for HIV-l-infected individuals. In addition, many of the Tar and Rev epitopes were shown to overlap with regions having functional activity or with regions previously identified as T-cell epitopes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 10:15:26