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Titolo:
ESCHERICHIA-COLI BACTERIOPHAGE-T1 DNA METHYLTRANSFERASE APPEARS TO INTERACT WITH ESCHERICHIA-COLI ENOLASE
Autore:
GASSNER C; SCHNEIDERSCHERZER E; LOTTSPEICH F; SCHWEIGER M; AUER B;
Indirizzi:
INNSBRUCK UNIV,INST BIOCHEM,PETER MAYR STR 1A A-6020 INNSBRUCK AUSTRIA INNSBRUCK UNIV,INST BIOCHEM A-6020 INNSBRUCK AUSTRIA MAX PLANCK INST BIOCHEM D-82152 MARTINSRIED GERMANY FREE UNIV BERLIN DAHLEM,INST BIOCHEM D-14195 BERLIN 33 GERMANY
Titolo Testata:
Biological chemistry
fascicolo: 4-5, volume: 379, anno: 1998,
pagine: 621 - 623
SICI:
1431-6730(1998)379:4-5<621:EBDMAT>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
METHYLATION; SEQUENCE; TRANSPOSITION; VIRUS-T1; IS50;
Keywords:
HIS-TAGGED EXPRESSION; ENZYME KINETICS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
C. Gassner et al., "ESCHERICHIA-COLI BACTERIOPHAGE-T1 DNA METHYLTRANSFERASE APPEARS TO INTERACT WITH ESCHERICHIA-COLI ENOLASE", Biological chemistry, 379(4-5), 1998, pp. 621-623

Abstract

Infection of Escherichia coli cells with bacteriophage T1 induces synthesis of a bacteriophage-specific DNA methyltransferase (M.EcoT1, EC No: 2.1.1.72) with a specificity for adenine residues in the sequence 5'GATC-3'. Purification of M.EcoT1 allowed the determination of the coding sequence of the gene (Schneider-Scherzer et al., 1990). The peptide of the entire coding sequence was over-expressed as a histidine-hexapeptide tagged protein in E. coli. Affinity purification using a Ni2chelating (Ni-NTA) resin yielded a recombinant enzyme with almost thesame enzymatic properties as the protein purified from T1 infected E.coli cells. Interestingly, in both purification procedures, a proteinwith a molecular weight of 50000 was found to copurify with M.EcoT1. The N-terminal amino acid sequence identified these proteins in both cases as E. coli enolase (EC No: 4.2.1.11).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 21:45:35