Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
RANDOMLY AMPLIFIED POLYMORPHIC DNA GENOTYPING OF SEROGROUP-A MENINGOCOCCI YIELDS RESULTS SIMILAR TO THOSE OBTAINED BY MULTILOCUS ENZYME ELECTROPHORESIS AND REVEALS NEW GENOTYPES
Autore:
BART A; SCHUURMAN IGA; ACHTMAN M; CAUGANT DA; DANKERT J; VANDERENDE A;
Indirizzi:
UNIV AMSTERDAM,ACAD MED CTR,DEPT MED MICROBIOL NL-1105 AZ AMSTERDAM NETHERLANDS MAX PLANCK INST MOL GENET D-14195 BERLIN GERMANY NATL INST PUBL HLTH,DEPT BACTERIOL,WORLD HLTH ORG COLLABORATING CTR REFERENCE & RES N-0403 OSLO NORWAY
Titolo Testata:
Journal of clinical microbiology
fascicolo: 6, volume: 36, anno: 1998,
pagine: 1746 - 1749
SICI:
0095-1137(1998)36:6<1746:RAPDGO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEISSERIA-MENINGITIDIS; DISEASE; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
A. Bart et al., "RANDOMLY AMPLIFIED POLYMORPHIC DNA GENOTYPING OF SEROGROUP-A MENINGOCOCCI YIELDS RESULTS SIMILAR TO THOSE OBTAINED BY MULTILOCUS ENZYME ELECTROPHORESIS AND REVEALS NEW GENOTYPES", Journal of clinical microbiology, 36(6), 1998, pp. 1746-1749

Abstract

Randomly amplified polymorphic DNA (RAPD) genotyping was applied to one representative strain of each of the 84 electrophoretic types (ETs)of Neisseria meningitidis serogroup A previously defined by multilocus enzyme electrophoresis (MEE) (J.-F. Wang et al., Infect. Immun. 60:5267-5282, 1992). Twenty-seven additional isolates comprising six ETs were also tested. MEE and RAPD genotyping yielded similar dendrograms at the subgroup level. Similar results were obtained by both methods for 18 serogroup A meningococci isolated in The Netherlands between 1989and 1993. Ten of these isolates defined a new subgroup, designated subgroup IX. One isolate belonged to the ET-5 complex, normally associated with serogroup B strains (D. A. Caugant et al., Proc. Natl. Acad. Sci. USA 83:4927-4931, 1986). By RAPD genotyping, meningococci can be linked to previously characterized genotypes by using a computerized database, and dendrograms based on cluster analyses can easily be generated. RAPD analysis offers advantages over MEE since intermediate numbers of isolates of serogroup A meningococci can quickly be assigned to known subgroups and new subgroups can be defined.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 06:49:06