Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DIVERSE AND RELATED 16S RIBOSOMAL-RNA-ENCODING DNA-SEQUENCES IN PROSTATE TISSUES OF MEN WITH CHRONIC PROSTATITIS
Autore:
RILEY DE; BERGER RE; MINER DC; KRIEGER JN;
Indirizzi:
UNIV WASHINGTON,SCH MED,DEPT UROL,PACIFIC AVE SEATTLE WA 98195 UNIV WASHINGTON,SCH MED,DEPT PATHOBIOL SEATTLE WA 98195 UNIV WASHINGTON,SCH PUBL HLTH,DEPT UROL SEATTLE WA 98195 UNIV WASHINGTON,SCH PUBL HLTH,DEPT PATHOBIOL SEATTLE WA 98195
Titolo Testata:
Journal of clinical microbiology
fascicolo: 6, volume: 36, anno: 1998,
pagine: 1646 - 1652
SICI:
0095-1137(1998)36:6<1646:DAR1RD>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC IDIOPATHIC PROSTATITIS; UNCULTURED MICROORGANISMS; UREAPLASMA-UREALYTICUM; RIBOSOMAL-RNA; AEROMONAS; PATHOGENS; IDENTIFICATION; INFECTIONS; CULTURE; TRACT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
D.E. Riley et al., "DIVERSE AND RELATED 16S RIBOSOMAL-RNA-ENCODING DNA-SEQUENCES IN PROSTATE TISSUES OF MEN WITH CHRONIC PROSTATITIS", Journal of clinical microbiology, 36(6), 1998, pp. 1646-1652

Abstract

Treatment of chronic prostatitis/chronic pelvic pain syndrome is often empirical because clinical culture methods fail to detect prostate-associated pathogens in >90% of patients. Previously, we tested 3 variety of specific-microorganism PCRs and began a DNA sequence study afterwe found that 77% of prostatitis patients were PCR positive for prokaryotic rRNA-encoding DNA sequences (rDNAs) despite negative cultures using optimal techniques. In the present study, 36 rDNA clones from 23 rDNA-positive patients,were sequenced. This study represents more thantwice the total rDNA sequence and more than twice the number of patients in the previous study, The increased number of patients and clonessequenced allowed enhanced phylogenetic analyses and refinements in our view of rDNA species inhabiting the prostate. A continuum of related rDNAs that might be arbitrarily described as two major groups of rDNAs and several minor groups was found. Sequences termed Pros A, identified in 8 (35%) of 23 rDNA-positive patients, grouped with Aeromonas,las spp. in phylogenetic studies, Sequences termed Pros B, identified in 17 (74%) of 23 rDNA-positive patients, were distinct from previouslyreported sequences, although all were >90% similar to known gram-negative bacteria. Of the nine patients for whom multiple rDNAs were sequenced, six had biopsy specimens containing rDNAs from more than one species. Four (17%) patients had rDNAs different from those of the Pros Aand Pros B groups. Of these four, one patient had rDNA similar to that of Flavobacterium spp., another had rDNA similar to that of Pseudomonas testosteroni, and two patients had rDNAs <70% similar to known rDNAs. These findings suggest that the prostate can harbor bacteria undetectable by traditional approaches. Most of these diverse sequences arenot reported in environments outside the prostate. The sequence similarities suggest adaptation of limited groups of bacteria to the microenvironment of the prostate. Further studies may elucidate the relationship of prostate-associated bacteria to chronic prostatitis/chronic pelvic pain syndrome.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:43:00