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Titolo:
DIFFERENTIAL EXPRESSION OF RAT AND HUMAN TYPE-1 METABOTROPIC GLUTAMATE-RECEPTOR SPLICE VARIANT MESSENGER-RNAS
Autore:
BERTHELE A; LAURIE DJ; PLATZER S; ZIEGLGANSBERGER W; TOLLE TR; SOMMER B;
Indirizzi:
NOVARTIS PHARMA AG CH-4002 BASEL SWITZERLAND NOVARTIS PHARMA AG CH-4002 BASEL SWITZERLAND MAX PLANCK INST PSYCHIAT D-8000 MUNICH GERMANY
Titolo Testata:
Neuroscience
fascicolo: 3, volume: 85, anno: 1998,
pagine: 733 - 749
SICI:
0306-4522(1998)85:3<733:DEORAH>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; CENTRAL-NERVOUS-SYSTEM; AMINO-ACID RECEPTORS; MGLUR1 MUTANT MICE; CELLULAR-LOCALIZATION; BRAIN; CLONING; HIPPOCAMPUS; DEPRESSION; THALAMUS;
Keywords:
IN SITU HYBRIDIZATION; METABOTROPIC GLUTAMATE RECEPTOR TYPE I; SPLICE VARIANTS; RAT; HUMAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
A. Berthele et al., "DIFFERENTIAL EXPRESSION OF RAT AND HUMAN TYPE-1 METABOTROPIC GLUTAMATE-RECEPTOR SPLICE VARIANT MESSENGER-RNAS", Neuroscience, 85(3), 1998, pp. 733-749

Abstract

The type I metabotropic glutamate receptor (mGlu(1)) messenger RNA and protein are known to be widely expressed in rat brain, but knowledgeof the regional expression of splice variants other than mGlu(1a) is limited. Probes were designed for in situ hybridization that specifically recognize each of the carboxy-terminal splice variants mGlu(1a), -(1b), -(1c) and -(1d). The novel rat mGlu(1d) sequence was obtained bypolymerase chain reaction and the predicted protein is highly homologous to the human sequence but contains both conservative and radical substitutions and is slightly longer (912 vs 908 amino acids). Each ratmGlu(1) splice variant messenger RNA was found in a unique expressionpattern. The messenger RNA encoding mGlu(1a) was abundant in cerebellar Purkinje cells and in mitral and tufted cells of the olfactory bulb. Strong expression was also detected in hippocampal interneurons, andneurons of the thalamus and substantia nigra, while moderate expression was found in colliculi and cerebellar granule cells. The mGlu(1b) messenger RNA was strongly expressed in Purkinje cells, hippocampal pyramidal neurons, dentate gyrus granule cells and lateral septum, and moderately expressed in striatal, superficial cortical and cerebellar granule neurons. The mGlu(1d) messenger RNA was expressed in all regionswhere mGlu(1a) and -(1b) were detected; abundant in Purkinje cells, mitral and tufted cells, and hippocampal principal neurons and interneurons, strong in thalamus and substantia nigra, and moderate in lateralseptum, cortex, striatum and colliculi. Human mGlu(1) splice variant expression in the cerebellum matched that found for the rat. No specific signal was found with a probe capable of hybridizing to the rat mGlu(1c) splice junction, although another probe designed against a more 3' sequence of mGlu(1c) gave strong signals in the cerebellum and hippocampus, and moderate signals in thalamus and colliculi. It is concluded that mGlu(1d) messenger RNA is widely expressed, that mGlu(1a) and -(1b) messenger RNAs are expressed in almost complementary patterns and that formation of the mGlu(1c) splice junction is a rare event. (C) 1998 IBRO. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 18:19:12