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Titolo:
STABILITY OF PITYROSPORUM-OVALE ALLERGENS DURING STORAGE
Autore:
LINTU P; SAVOLAINEN J; KALIMO K; TERHO EO;
Indirizzi:
UNIV TURKU,DEPT CLIN ALLERGOL,MEDICITY,TYKISTONKATU 6 FIN-25020 TURKUFINLAND UNIV TURKU,DEPT PULM DIS FIN-25020 TURKU FINLAND UNIV TURKU,DEPT DERMATOL FIN-25020 TURKU FINLAND UNIV TURKU,DEPT MED MICROBIOL,TURKU ALLERGY RES UNIT,MEDICITY RES LABFIN-25020 TURKU FINLAND HOP ARNAUD VILLENEUVE,INSERM MONTPELLIER FRANCE
Titolo Testata:
Clinical and experimental allergy
fascicolo: 4, volume: 28, anno: 1998,
pagine: 486 - 490
SICI:
0954-7894(1998)28:4<486:SOPADS>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANDIDA-ALBICANS; POLLEN EXTRACT; DERMATITIS; PROTEINS;
Keywords:
ALLERGEN; PITYROSPORUM OVALE; STABILITY; SKIN-PRICK TEST; YEAST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
P. Lintu et al., "STABILITY OF PITYROSPORUM-OVALE ALLERGENS DURING STORAGE", Clinical and experimental allergy, 28(4), 1998, pp. 486-490

Abstract

Background. Pityrosporum ovale is a common saprophyte on the skin capable to induce IgE antibody production in atopic dermatitis. GenerallyIgE response to P. ovale has been established with skin-prick test, but the stability of P. ovale skin test allergens is not known. Objective and Methods. In this study we analysed the stability of identified allergens of P. ovale in skin test solutions during storage. The stability was checked with immunoblotting and densitometry after different time periods and at various temperatures. Results. Coomassie Brilliantblue staining clearly showed, that proteins were preserved poorly at 20 degrees C in 50% glycerol. Even extracts stored at + 4 degrees C during 6 and 12 months in 50% glycerol were degraded. Only extract stored at + 4 degrees C for 1 month in 50% glycerol was guite well preserved. According to densitometry results the 9 kDa band was the most stable main protein allergen. The 20 kDa and expecially the 96 kDa bands were far more labile. The stability of low molecular allergens was in general better than the stability of high molecular weight allergens. Conclusions. The stability of P. ovale extracts stored for more than 1month was poor even when kept at + 4 degrees C temperature. Reliability of negative skin prick results should be questioned when older extracts are used. In skin test negative cases, the sensitization should be evaluated also with the measurement of specific serum IgE antibodiesto P. ovale.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 16:00:30