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Titolo:
OLIVE POLLEN ALLERGY - SEARCHING FOR IMMUNODOMINANT T-CELL EPITOPES ON THE OLE-E-1 MOLECULE
Autore:
CARDABA B; DELPOZO V; JURADO A; GALLARDO S; CORTEGANO I; ARRIETA I; DELAMO A; TRAMON P; FLORIDO F; SASTRE J; PALOMINO P; LAHOZ C;
Indirizzi:
FDN JIMENEZ DIAZ,DEPT IMMUNOL,AVDA REYES CATOLICOS 2 E-28040 MADRID SPAIN FDN JIMENEZ DIAZ,DEPT IMMUNOL E-28040 MADRID SPAIN HOSP GEN ESPECIALIDADES,DEPT ALLERGY JAEN SPAIN FDN JIMENEZ DIAZ,DEPT ALLERGY E-28040 MADRID SPAIN
Titolo Testata:
Clinical and experimental allergy
fascicolo: 4, volume: 28, anno: 1998,
pagine: 413 - 422
SICI:
0954-7894(1998)28:4<413:OPA-SF>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-SEQUENCE; MAJOR ALLERGEN; E-I; EUROPEA POLLEN; CROSS-REACTIVITY; OLEACEAE POLLENS; P-I; SPECIFICITY; LOCALIZATION; RESPONSES;
Keywords:
OLE E 1; HLA CLASS II; DR7/DQ2; T-CELL EPITOPES; OLIVE POLLEN SENSITIZATION; MAIN ALLERGEN OF OLIVE POLLEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
B. Cardaba et al., "OLIVE POLLEN ALLERGY - SEARCHING FOR IMMUNODOMINANT T-CELL EPITOPES ON THE OLE-E-1 MOLECULE", Clinical and experimental allergy, 28(4), 1998, pp. 413-422

Abstract

Background. The amino-acid and nucleotide sequence of Ole e 1 (the major antigen of olive pollen) has been described and the IgE antibody response to this major allergen was associated with DR7/DQ2 antigens. With this previous data we try to define the T-cell epitopes implicatedin Ole e 1 reactivity. Objectives. To study the recognition of T cells (derived from allergic and non-allergic Ole e 1 patients) to Ole e 1synthetic peptides in order to define immunodominant T-cell epitopes. Methods. We have compared the proliferative response of the peripheral blood mononuclear cells from Ole e 1 sensitized patients vs. non-sensitized controls, induced by 14 Ole e 1 synthetic peptides. Thirty subjects were classified in two groups: group 1 (nonresponders against Ole e 1, n = 16) and group 2(Ole e 1 responders, n = 14), according to their clinical parameters and the presence or not in their sera of the significant Ole e 1 IgE antibody levels. Results. Our results shown that it is possible to find T cells reactive to Ole e 1 peptides in patients with and without significant levels of Ole e 1 IgE antibodies. However, the percentage of response was higher in patients with IgE antibodies 71.4% vs 25%), and the recognition profile was different: the control group showed a broad reactivity pattern, in contrast, the response by the 'Ole e 1 responders' group was mainly directed against three peptides of the carboxi-terminal region, peptides 10 (91-102), 12 (109-120) and 13 (119-130), with a response frequency of 35.7, 28.5 and 28.5%, respectively. By direct and inhibition test no antibody response was found against the synthetic peptides.Conclusions. Our data suggest that the regions between 91 and 102 and 109-130 aminoacids on the Ole e 1 molecule are immunodominant T-cell epitopes. These epitopes arenot recognized by IgE antibodies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 09:47:45