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Titolo:
THE ROLE OF GABAERGIC INPUTS FOR COINCIDENCE DETECTION IN THE NEURONSOF NUCLEUS LAMINARIS OF THE CHICK
Autore:
FUNABIKI K; KOYANO K; OHMORI H;
Indirizzi:
KYOTO UNIV,FAC MED,DEPT PHYSIOL KYOTO 6068501 JAPAN KYOTO UNIV,FAC MED,DEPT PHYSIOL KYOTO 6068501 JAPAN KYOTO UNIV,FAC MED,DEPT OTOLARYNGOL KYOTO 6068501 JAPAN
Titolo Testata:
Journal of physiology
fascicolo: 3, volume: 508, anno: 1998,
pagine: 851 - 869
SICI:
0022-3751(1998)508:3<851:TROGIF>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
STEM AUDITORY NUCLEI; INTERAURAL TIME DIFFERENCES; MEDIAL SUPERIOR OLIVE; BRAIN-STEM; COCHLEAR NUCLEUS; N-LAMINARIS; IN-VITRO; MAGNOCELLULARIS; GABA; ORGANIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
K. Funabiki et al., "THE ROLE OF GABAERGIC INPUTS FOR COINCIDENCE DETECTION IN THE NEURONSOF NUCLEUS LAMINARIS OF THE CHICK", Journal of physiology, 508(3), 1998, pp. 851-869

Abstract

1. Synaptic inputs to nucleus laminaris (NL) neurones were studied ina brainstem slice preparation of chick embryos (E15-20) using the whole-cell patch clamp technique. NL neurones are third order auditory neurones and are proposed to behave as coincidence detectors concerned viith interaural timing discrimination. 2. Under voltage clamp conditions, electrical stimuli applied to either ventral or dorsal dendritic layers evoked EPSCs. These fast currents decayed with a time constant of 1.1 ms near the resting potential, reversed close to 0 mV, and were blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 20 mu M) or 6,7-dinitro-quinoxaline-2,3-dione (DNQX, 20 mu M). Coincident or near coincident stimulation of the ventral and the dorsal dendritic layers increased the probability of action potential generation (response probability). 3. Ln the presence of CNQX (40 mu M) other postsynaptic currents (PSCs) were observed, which reversed close to the equilibrium potential for chloride (E-Cl), and were reversibly blocked by bicuculline (20 mu M) and, therefore, were mediated by GABA, receptors. SpontaneousGABAergic PSCs were inward going near the resting membrane potential immediately after starting whole-cell recording with a low Cl- (5 mM, E-Cl = -90 mV) pipette medium, but became outward-going with time. This indicates that GABAergic inputs may generate depolarizing potentialsin intact NL neurones. 4. Local GABA (10 mu M) application reduced both the EPSP and EPSC amplitude and shortened the EPSP decay time constant (from 5.3 to 1.2 ms), while the EPSC decay time constant wets not affected (from 1.3 to 1.2 ms). These GABA effects were mostly due to the shunting conductance of the postsynaptic GABA, receptors. 5. Depolarizing current injections combined with electrical stimuli to a unilateral axon bundle simulated bilateral synaptic inputs. Response probability increased with decreased interstimulus intervals, while local GABA (10 mu M) application to the soma narrowed the time dependence of the response probability.6. These results suggest that GABAergic inputs to NL neurones may serve to improve coincidence detection of the bilateral excitatory inputs through an increase in membrane conductance.

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Documento generato il 05/12/20 alle ore 01:44:31