Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
SELECTION IN-VITRO OF NOVEL RIBOZYMES FROM A PARTIALLY RANDOMIZED U2 AND U6 SNRNA LIBRARY
Autore:
TUSCHL T; SHARP PA; BARTEL DP;
Indirizzi:
WHITEHEAD INST BIOMED RES BOSTON MA 02114 WHITEHEAD INST BIOMED RES BOSTON MA 02114 MIT,CTR CANC RES CAMBRIDGE MA 02139 MIT,DEPT BIOL CAMBRIDGE MA 02139
Titolo Testata:
EMBO journal
fascicolo: 9, volume: 17, anno: 1998,
pagine: 2637 - 2650
SICI:
0261-4189(1998)17:9<2637:SIONRF>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCISED INTERVENING SEQUENCE; RNA SPLICING INVITRO; REVERSE-TRANSCRIPTASE; DEBRANCHING ENZYME; HAIRPIN RIBOZYMES; CLEAVAGE; TEMPLATE; INTRON; POLYMERASE; EVOLUTION;
Keywords:
IN VITRO SELECTION; REVERSE TRANSCRIPTASE; RIBOZYME; RNA BRANCH; SNRNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
T. Tuschl et al., "SELECTION IN-VITRO OF NOVEL RIBOZYMES FROM A PARTIALLY RANDOMIZED U2 AND U6 SNRNA LIBRARY", EMBO journal, 17(9), 1998, pp. 2637-2650

Abstract

Combinatorial libraries related to spliceosomal U2 and U6 snRNAs weretested for catalytic reactions typical of the splicing of nuclear pre-mRNAs, Ribozymes with four different activities were selected based on covalent bond formation to a substrate RNA. The first activity was reversible self-cleavage; ribozymes self-cleaved then ligated the 5'-hydroxyl group of the substrate oligonucleotide to their 2',3'-cyclic phosphate intermediate. The second activity was 2',5'-branch formation by the attack of a substrate 2'-hydroxyl group on the 5'-terminal triphosphate of the ribozyme transcript, releasing pyrophosphate. The thirdribozyme activity was similar to reversible self-cleavage but was a three-step reaction, This ribozyme self-cleaved, then cleaved the substrate in trans, and then ligated the substrate 3' cleavage product to its cyclic phosphate intermediate. This three-step pathway shares similarities with the pathway of tRNA splicing. The fourth activity was 2',3'-branch formation; to form this unusual branch, a 2'-hydroxyl of thesubstrate attacked an internal phosphate of the ribozyme, releasing an oligonucleotide leaving group, The isolation of branching activitiesby the in vitro selection protocol was unanticipated and was due to surprising properties of reverse transcriptase, which can read through 2',5'- or 2',3'-branches and efficiently perform non-templated intramolecular jumps.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 19:03:57