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Titolo:
THE GENE STRUCTURE AND ORGANIZATION OF MOUSE PG-LB, A SMALL CHONDROITIN DERMATAN SULFATE PROTEOGLYCAN
Autore:
IWATA Y; SHINOMURA T; KURITA K; ZAKO M; KIMATA K;
Indirizzi:
AICHI MED UNIV,INST MOL SCI MED NAGAKUTE AICHI 48011 JAPAN AICHI MED UNIV,INST MOL SCI MED NAGAKUTE AICHI 48011 JAPAN NAGOYA UNIV,SCH MED,DEPT ORTHOPAED SURG NAGOYA AICHI 466 JAPAN
Titolo Testata:
Biochemical journal
, volume: 331, anno: 1998,
parte:, 3
pagine: 959 - 964
SICI:
0264-6021(1998)331:<959:TGSAOO>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPLETE CDNA CLONING; SULFATE PROTEOGLYCAN; DNA-BINDING; EPIPHYSEAL CARTILAGE; GENOMIC ORGANIZATION; MOLECULAR-CLONING; BIGLYCAN GENE; PROTEIN; DECORIN; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
Y. Iwata et al., "THE GENE STRUCTURE AND ORGANIZATION OF MOUSE PG-LB, A SMALL CHONDROITIN DERMATAN SULFATE PROTEOGLYCAN", Biochemical journal, 331, 1998, pp. 959-964

Abstract

PG-Lb was originally characterized as a small chondroitin/dermatan sulphate proteoglycan expressed preferentially in the zones of flattenedchondrocytes in developing chick limb cartilage. The occurrence of this proteoglycan in mammalian cartilage has been shown by the isolationof a cDNA clone from mouse cartilage cDNA library [Kurita, Shinomura,Ujita, Zako, Kida, Iwata and Kimata (1996) Biochem. J. 318, 909-914]. To understand the regulation mechanisms for such a unique expression,we have investigated a genomic DNA structure of the PG-Lb gene. The gene is composed of seven exons and six introns spanning more than 50 kb. The leucine-rich repeats are encoded from exon V to exon VII. The transcription initiation site has been determined by rapid amplification of the cDNA ends ('5'-RACE'). The possible TATA box was detected about 90 bp upstream of the adenosine residue that was numbered as position + 1. Further analyses of 1.5 kb of the 5' flanking region and 2.2 kb of the first intron have revealed several potential binding motifs for transcription factors such as Sox 5 and 9. The presence of those sequences in the PG-Lb gene was discussed in relation to the unique expression of this proteoglycan. The chromosomal localization of the murine PG-Lb gene was determined to be on the mouse chromosome 10 by the fluorescence-in-situ-hybridization ('FISH') method.

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Documento generato il 06/04/20 alle ore 08:31:29