Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
RELATIONSHIPS BETWEEN DOPAMINE-INDUCED CHANGES IN CYTOSOLIC-FREE CALCIUM-CONCENTRATION ([CA2- ELEVATED [CA2+](I) DOES NOT INDICATE PROLACTIN-RELEASE(](I)) AND RATE OF PROLACTIN SECRETION )
Autore:
CHANG A; SHIN SH;
Indirizzi:
QUEENS UNIV,DEPT PHYSIOL,BOTTERELL HALL KINGSTON ON K7L 3N6 CANADA QUEENS UNIV,DEPT PHYSIOL KINGSTON ON K7L 3N6 CANADA
Titolo Testata:
ENDOCRINE
fascicolo: 3, volume: 7, anno: 1997,
pagine: 343 - 349
SICI:
1355-008X(1997)7:3<343:RBDCIC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTERIOR-PITUITARY-CELLS; TRAPPED FLUORESCENT INDICATOR; HORMONE-RELEASE; POTASSIUM CHANNELS; LACTOTROPH CELLS; RECEPTORS; CURRENTS; STIMULATION; INHIBITION; G(I-ALPHA-3);
Keywords:
DOPAMINE; CYTOSOLIC CALCIUM; PROLACTIN; FLUO-3; GH(4)ZR(7);
Tipo documento:
Article
Natura:
Periodico
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
A. Chang e S.H. Shin, "RELATIONSHIPS BETWEEN DOPAMINE-INDUCED CHANGES IN CYTOSOLIC-FREE CALCIUM-CONCENTRATION ([CA2- ELEVATED [CA2+](I) DOES NOT INDICATE PROLACTIN-RELEASE(](I)) AND RATE OF PROLACTIN SECRETION )", ENDOCRINE, 7(3), 1997, pp. 343-349

Abstract

This study was undertaken to investigate the relationship between dopamine (DA) induced changes in the cytosolic calcium concentration ([Ca2+](i)) and the rate of prolactin secretion using GH(4)ZR(7), a rat pituitary cell line, which express only one subtype of D-2 receptor. GH(4)ZR(7) cells were loaded with Fluo-3, a fluorescent Ca2+ indicator, and then perifused with two different doses of DA (10(-7) mol/L and 5 x10(-4)mol/L). We monitored changes in [Ca2+](i) and rate of prolactinrelease simultaneously by attaching a spectrofluorometer to a dynamicperifusion system. DA has stimulatory and inhibitory effect on prolactin secretion in GH(4)ZR(7) cells; 10(-7) mol/L DA slightly increased [Ca2+](i) and stimulated prolactin release, whereas 5 x 10(-4) mol/L DA decreased [Ca2+](i) and inhibited prolactin secretion, When the cells were pretreated with pertussis toxin (PTX), 10(-7) mol/L DA had no significant change in [Ca2+](i) while stimulating prolactin release, and 5 x 10(-4) mol/L DA reduced [Ca2+](i) without having any significanteffect on the rate of prolactin secretion. The results of this study demonstrate that changes in [Ca2+](i) do not always correlate with therate of prolactin release from lactotrophs. The dissociation between [Ca2+](i) and prolactin release is somewhat expected considering the diverse role of [Ca2+](i) and post-[Ca2+](i) events, which can change the rate of prolactin release.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 16:03:55