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Titolo:
PREVENTION OF CELLULAR EDEMA DIRECTLY CAUSED BY HYPOTHERMIC CARDIOPLEGIA - STUDIES IN ISOLATED HUMAN AND RABBIT ATRIAL MYOCYTES
Autore:
SHAFFER RF; BAUMGARTEN CM; DAMIANO RJ;
Indirizzi:
PENN STATE UNIV,DIV CARDIOTHORAC SURG,POB 850 HERSHEY PA 17033 PENN STATE UNIV,DIV CARDIOTHORAC SURG HERSHEY PA 17033 VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT PHYSIOL RICHMOND VA23298 VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT SURG RICHMOND VA 23298
Titolo Testata:
Journal of thoracic and cardiovascular surgery
fascicolo: 5, volume: 115, anno: 1998,
pagine: 1189 - 1195
SICI:
0022-5223(1998)115:5<1189:POCEDC>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
VENTRICULAR MYOCYTES; GLOBAL-ISCHEMIA; HUMAN-HEART; RAT-HEART; ARREST; VOLUME; CONSEQUENCES; OUABAIN; DAMAGE; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
R.F. Shaffer et al., "PREVENTION OF CELLULAR EDEMA DIRECTLY CAUSED BY HYPOTHERMIC CARDIOPLEGIA - STUDIES IN ISOLATED HUMAN AND RABBIT ATRIAL MYOCYTES", Journal of thoracic and cardiovascular surgery, 115(5), 1998, pp. 1189-1195

Abstract

Objectives: This study tested the hypothesis that edema during hypothermic cardioplegia is caused by the hypotonicity of the perfusate at cold temperatures. Methods: The volume of isolated human and rabbit atrial myocytes was measured by video microscopy under nonischemic conditions. Each cell served as its own control. Results: After equilibration in 37 degrees C physiologic buffer (Tyrode's solution), exposure to 9 degrees C St. Thomas' Hospital solution for 20 minutes caused human atrial cells to swell by 20% and rabbit atrial cells to swell by 10%. Cell volume fully recovered on rewarming in 37 degrees C physiologic solution. Cell swelling was due to the composition of St. Thomas' Hospital solution rather than hypothermia alone. Exposure to 9 degrees C physiologic solution did not significantly affect cell volume. Swelling of myocytes was largely prevented by replacing most of the Cl- in St. Thomas' Hospital solution with an impermeant anion so that the productof the concentrations of K+ and Cl- were the same as in the physiologic solution. Conclusions: This study suggests that cell swelling during hypothermic cardioplegia is caused in part by the composition of thecardioplegic solution. The volume of cardiac myocytes appears to follow a Donnan equilibrium in the cold, and the perfusate KCl product determines water movement. Thus, the tonicity of hyperkalemic cardioplegic solutions can be adjusted to a physiologic value by replacing most Cl- by an impermeant anion. Following this simple principle, a reformulation of cardioplegic solutions may be able to minimize iatrogenic myocardial edema.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 07:11:25