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Titolo:
DIRECT MICROCALORIMETRY AS A TECHNIQUE IN CELL-CULTURES
Autore:
BOTTCHER H; FURST P;
Indirizzi:
UNIV HOHENHEIM,INST BIOL CHEM & NUTR 140 D-70593 STUTTGART GERMANY
Titolo Testata:
Bailliere's clinical endocrinology and metabolism
fascicolo: 4, volume: 11, anno: 1997,
pagine: 739 - 752
SICI:
0950-351X(1997)11:4<739:DMAATI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-LYMPHOMA CELLS; SODIUM-POTASSIUM PUMP; HEAT-PRODUCTION RATE; NA-K PUMP; HUMAN ERYTHROCYTES; HYPERTHYROID PATIENTS; HEPATIC TISSUE; METABOLISM; THERMOGENESIS; LEUKEMIA;
Keywords:
DRUG; ENERGY METABOLISM; HEAT PRODUCTION; HORMONE; MICROCALORIMETRIC; MICROCATHETER; PERFUSION/TITRATION; SUBSTRATE UTILIZATION; THERMOGENESIS; THERMOPILE HEAT CONDUCTION CALORIMETER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
H. Bottcher e P. Furst, "DIRECT MICROCALORIMETRY AS A TECHNIQUE IN CELL-CULTURES", Bailliere's clinical endocrinology and metabolism, 11(4), 1997, pp. 739-752

Abstract

In vitro studies of energy metabolism in isolated cells contribute toimproved knowledge of human energy metabolism under normal and pathological conditions. In every cellular system energy is taken up, metabolized and finally transformed into heat, which is dissipated into the environment. Thus, energy turnover of isolated cells can be assessed by microcalorimetric determination of their heat production. Microcalorimeters of the thermopile heat conduction type facilitate direct physical determination of thermogenesis with a sensitivity of 0.2 mu W; 10(4)-10(5) cells being sufficient for one measurement. Peltier elements are sandwiched between the sample and a precisely thermostated heat sink, creating a detectable voltage proportional to the heat production. For adequate interpretation of the results, simultaneous biochemical investigations of relevant metabolic pathways are required. Up to now,numerous studies with blood cells, skeletal and heart muscle cells, hepatocytes, endothelial cells, fibroblasts and adipocytes have been performed in relation to various diseases and under the influence of certain hormones and pharmacological agents.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 22:43:43