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Titolo:
ACCUMULATION OF ACETYLCHOLINE-RECEPTORS IS A NECESSARY CONDITION FOR NORMAL ACCUMULATION OF ACETYLCHOLINESTERASE DURING IN-VITRO NEUROMUSCULAR SYNAPTOGENESIS
Autore:
DELAPORTE S; CHAUBOURT E; FABRE F; POULAS K; CHAPRON J; EYMARD B; TZARTOS S; KOENIG J;
Indirizzi:
CNRS UPR 9040,LAB NEUROBIOL CELLULAIRE & MOL GIF SUR YVETTE FRANCE UNIV BORDEAUX 2,LAB NEUROBIOL CELLULAIRE,CNRS URA 1126 TALENCE FRANCE HELLENIC PASTEUR INST,DEPT BIOCHEM ATHENS GREECE CTR CHIRURG MARIE LANNELONGUE,CNRS URA 1159,IMMUNOL LAB LE PLESSIS ROBINS FRANCE
Titolo Testata:
European journal of neuroscience
fascicolo: 5, volume: 10, anno: 1998,
pagine: 1631 - 1643
SICI:
0953-816X(1998)10:5<1631:AOAIAN>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAIN IMMUNOGENIC REGION; AGRIN-LIKE MOLECULES; COLLAGEN-TAILED ACETYLCHOLINESTERASE; DYSTROPHIN-GLYCOPROTEIN COMPLEX; ALPHA-BUNGAROTOXIN BINDING; NERVE-MUSCLE CULTURES; MYASTHENIA-GRAVIS; EXTRACELLULAR-MATRIX; MESSENGER-RNA; DEVELOPMENTAL REGULATION;
Keywords:
AGRIN; C2 VARIANT; MICE; NEUROMUSCULAR-JUNCTION; RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
81
Recensione:
Indirizzi per estratti:
Citazione:
S. Delaporte et al., "ACCUMULATION OF ACETYLCHOLINE-RECEPTORS IS A NECESSARY CONDITION FOR NORMAL ACCUMULATION OF ACETYLCHOLINESTERASE DURING IN-VITRO NEUROMUSCULAR SYNAPTOGENESIS", European journal of neuroscience, 10(5), 1998, pp. 1631-1643

Abstract

To study a step of the very complex processes of the formation of theneuromuscular junction (NMJ), we have analysed the clustering of acetylcholine receptors (AChR) and acetylcholinesterase (AChE) in myotubescultured in various conditions. On the surface of rat myotubes cultured in the presence of spinal cord cells from embryonic rat, numerous AChE clusters appeared. Such clusters are always co-localized with AChRclusters, but the reverse is not true: the number of AChR clusters largely exceeds that of AChE clusters. Very few AChE clusters formed when such cc-cultures were treated with monoclonal antibodies (mAbs) against the main immunogenic region (MIR) of the AChR, which provoke internalization and degradation of the AChRs of the muscular membrane. The total levels of AChE and proportions of molecular forms were unaffected. We also used non-innervated myotubes in which addition of agrin, a protein normally synthesized by motoneurons, transported to nerve terminals and inserted into the synaptic basal lamina, induces the formation of small clusters of AChE. When added to rat myotubes devoid of membrane AChR, agrin-induced AChE clusters did not form. Finally, we analysed the capacity of the variant of the C2 mouse muscle cell fine deficient in AChR (1R-) to form clusters of AChE in cc-cultures with spinal cord cells from rat: no formation of AChE clusters could be observed. In all these different systems of cultures, the conditions which prevented clustering of AChR (anti-AChR antibodies, deficiency of the variant C2 cell line) also suppressed AChE clustering, We concluded that clustering of AChR is a prerequisite for clustering of AChE, so that NMJ formation implies the sequential accumulation of these two components.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 22:09:39