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Titolo:
PURIFICATION AND CHARACTERIZATION OF AN INTRACELLULAR AMINOPEPTIDASE FROM BREVIBACTERIUM LINENS ATCC-9174
Autore:
RATTRAY FP; FOX PF;
Indirizzi:
NATL UNIV IRELAND UNIV COLL CORK,DEPT FOOD CHEM CORK IRELAND
Titolo Testata:
Le Lait
fascicolo: 1, volume: 77, anno: 1997,
pagine: 169 - 180
SICI:
0023-7302(1997)77:1<169:PACOAI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRACELLULAR PROTEINASE;
Keywords:
AMINOPEPTIDASE; BREVIBACTERIUM LINENS; CHEESE; PURIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
F.P. Rattray e P.F. Fox, "PURIFICATION AND CHARACTERIZATION OF AN INTRACELLULAR AMINOPEPTIDASE FROM BREVIBACTERIUM LINENS ATCC-9174", Le Lait, 77(1), 1997, pp. 169-180

Abstract

An intracellular aminopeptidase from Brevibacterium linens ATCC 9174 was purified 4300-fold to homogeneity using ammonium sulphate fractionation, anion exchange chromatography, hydrophobic interaction chromatography and anion exchange chromatography. The pH and temperature optima were 8.5 and 35 degrees C, respectively. The purified aminopeptidasewas stable over the range pH 8 to 10, and was thermally stable up to 20 degrees C at pH 8.5. The molecular mass of the enzyme was found to be 59 kDa by SDS-PAGE and 69 kDa by gel filtration, indicating that the native enzyme exists as a monomer. The aminopeptidase was strongly inhibited by the thiol blocking agent, p-hydroxymercuribenzoate, and byCo2+ and Zn2+; activity was unaffected by metal chelators, reducing agents or phenylmethylsulphonyl fluoride. K-m and k(cat) values for L-Ala-p-NA were 3.3 mmol/L and 4.3 s(-1), respectively, while the corresponding Values for L-Gly-p-NA were 0.2 mmol/L and 7.6 s(-1), respectively. The aminopeptidase hydrolysed dipeptides with an alanine residue at the N-terminal but tripeptides were not hydrolysed. The sequence of the first 19 N-terminal amino acids was p-Thr-Ala-Ala-Ile-Ile-Asp-Arg-Leu-?-Asn-Ala-?-Thr.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 20:52:52