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Titolo:
INTERNALIZATION WITH HIGH TARGETING POTENTIAL OF MOUSE MONOCLONAL-ANTIBODY ONS-M21 RECOGNIZING HUMAN-MALIGNANT GLIOMA ANTIGEN
Autore:
SHIMIZU K; PARK KC; TAMURA K; KISHIMA H; KAWATA H; YOSHIMURA Y; SEKIMORI Y; MIYAO Y; HAYAKAWA T;
Indirizzi:
OSAKA UNIV,SCH MED,DEPT NEUROSURG,2-2 YAMADAOKA SUITA OSAKA 565 JAPAN CHUGAI PHARMACEUT CO LTD,FUJI GOTEMBA RES LABS SHIZUOKA 412 JAPAN
Titolo Testata:
Cancer letters
fascicolo: 1-2, volume: 127, anno: 1998,
pagine: 171 - 176
SICI:
0304-3835(1998)127:1-2<171:IWHTPO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RADIATION-THERAPY; CYTO-TOXICITY; IMMUNOTOXINS; TUMORS; RICIN; CELLS; MEDULLOBLASTOMA; CHEMOTHERAPY; ENDOCYTOSIS; ASTROCYTOMA;
Keywords:
MONOCLONAL ANTIBODY; INTERNALIZATION; IMMUNOTOXIN; RICIN A; MALIGNANT GLIOMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
K. Shimizu et al., "INTERNALIZATION WITH HIGH TARGETING POTENTIAL OF MOUSE MONOCLONAL-ANTIBODY ONS-M21 RECOGNIZING HUMAN-MALIGNANT GLIOMA ANTIGEN", Cancer letters, 127(1-2), 1998, pp. 171-176

Abstract

In order to evaluate the targeting potential of mouse monoclonal antibody ONS-M21 recognizing a human astrocytoma-and medulloblastoma-associated antigen, the internalization ability of this antibody and the selective cytotoxicity in the toxin-conjugated form were examined. Internalization assay with I-125-labeled ONS-M21 showed that about 20% of the total radioactivities was detected in the cellular fraction of human medulloblastoma cell line ONS-76 cells and that the reaction reacheda plateau level in 30 min. To examine the selective delivery capacityof a high molecular substance in place of I-125, an immunotoxin was prepared with ricin A chain and ONS-M21 via disulfide bonds. A cytotoxic effect against ONS-76 cells was found with [H-3]thymidine incorporation assay using the immunotoxin, but not against antigen-negative HuH-7 and SW480 cells. These results suggest that ONS-M21 could effectively deliver toxins, chemotherapeutic agents or radionuclei to malignant glioma specifically. (C) 1998 Elsevier Science Ireland Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 14:59:50