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Titolo:
MEASURING PREHEPATIC INSULIN-SECRETION USING A POPULATION-MODEL OF C-PEPTIDE KINETICS - ACCURACY AND REQUIRED SAMPLING SCHEDULE
Autore:
HOVORKA R; KOUKKOU E; SOUTHERDEN D; POWRIE JK; YOUNG MA;
Indirizzi:
CITY UNIV LONDON,CTR MEASUREMENT & INFORMAT MED,METAB MODELLING GRP,NORTHAMPTON SQ LONDON EC1V 0HB ENGLAND UNITED MED & DENT SCH GUYS & ST THOMASS HOSP,ST THOMAS HOSP,DEPT ENDOCRINOL & DIABET,DIV MED LONDON SE1 7EH ENGLAND GLAXO WELLCOME RES & DEV LTD,DEPT PHARMACOKINET GREENFORD MIDDX ENGLAND
Titolo Testata:
Diabetologia
fascicolo: 5, volume: 41, anno: 1998,
pagine: 548 - 554
SICI:
0012-186X(1998)41:5<548:MPIUAP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSE-TOLERANCE TEST; INFUSION; HUMANS; RATES; PATTERN;
Keywords:
INSULIN SECRETION; C-PEPTIDE KINETICS; NORMAL SUBJECTS; TYPE 2 DIABETES; MATHEMATICAL MODELING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
R. Hovorka et al., "MEASURING PREHEPATIC INSULIN-SECRETION USING A POPULATION-MODEL OF C-PEPTIDE KINETICS - ACCURACY AND REQUIRED SAMPLING SCHEDULE", Diabetologia, 41(5), 1998, pp. 548-554

Abstract

The accuracy of calculations of pre-hepatic insulin secretion were investigated, to provide independent validation of a population model ofC-peptide kinetics. The effects of sampling frequency were also assessed. Five normal subjects (aged 28 to 43 years; BMI (kg/m(2)) 20.5 to 24.5) and five subjects with non-insulin-dependent diabetes mellitus (NIDDM) treated by diet alone (aged 34 to 57 years; BMI 22.6 to 25.6) were given a variable intravenous infusion of biosynthetic human C-peptide (BHCP) (t = -60 to 240 min) mimicking meal stimulated C-peptide secretion, with short-term oscillations (peak approximately every 12 min) superimposed on the infusion profile. Plasma C-peptide was measured every 5 min (t = 0 to 240 min). The BHCP infusion was reconstructed from C-peptide measurements using a population model of C-peptide kinetics and a deconvolution method. Bias, defined as the percentage difference between the total amount of calculated BHCP and the total amount of infused BHCP (t = 0 to 240 min), indicated that overall C-peptide secretion can be measured with 14% [95 % confidence interval (CI) -11 to39%] and 21% (95% CI -3 to 45 %) accuracy in normal subjects and subjects with NIDDM respectively. Accuracy was not reduced by reducing thesampling frequency to every 30 min. The root mean square error, measuring the average deviation between the infused and normalised calculated BHCP profiles, was also independent of the sampling frequency [mean(95 % CI) 0.9 (0.3 to 1.6) pmol/kg per min in normal subjects; 1.0 (0.9 to 1.1) pmol/kg per min in subjects with NIDDM]. Deconvolution employing a population model of C-peptide kinetics can be used to estimatepostprandial total C-peptide secretion with biases of 14% and 22% respectively in normal subjects and subjects with NIDDM. Plasma C-peptidesamples need only be drawn every 30 minutes.

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Documento generato il 04/07/20 alle ore 18:17:12