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Titolo:
POLYAMINES ARE REQUIRED FOR MICROTUBULE FORMATION DURING GASTRIC-MUCOSAL HEALING
Autore:
BANAN A; MCCORMACK SA; JOHNSON LR;
Indirizzi:
UNIV TENNESSEE,COLL MED,DEPT PHYSIOL & BIOPHYS,NASH RES BLDG,894 UNION AVE MEMPHIS TN 38163 UNIV TENNESSEE,COLL MED,DEPT PHYSIOL & BIOPHYS MEMPHIS TN 38163
Titolo Testata:
American journal of physiology: Gastrointestinal and liver physiology
fascicolo: 5, volume: 37, anno: 1998,
pagine: 879 - 885
SICI:
0193-1857(1998)37:5<879:PARFMF>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORNITHINE DECARBOXYLASE; PSEUDOPODIAL ACTIVITY; STRESS; ASPIRIN; SURFACE; DAMAGE; REPAIR;
Keywords:
COLCHICINE; GASTRIC ULCERS; HYPERTONIC NACL; ALPHA-DIFLUOROMETHYLORNITHINE; SPERMIDINE; ORNITHINE DECARBOXYLASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
A. Banan et al., "POLYAMINES ARE REQUIRED FOR MICROTUBULE FORMATION DURING GASTRIC-MUCOSAL HEALING", American journal of physiology: Gastrointestinal and liver physiology, 37(5), 1998, pp. 879-885

Abstract

Polyamines are essential for the repair of gastric and duodenal erosions. Concentrated NaCl (3.4 M) given intragastrically damages the oxyntic gland mucosa and increases the activity of gastric mucosal ornithine decarboxylase (ODC), the first rate-limiting enzyme in polyamine synthesis. The nature of the process of restitution of damaged mucosa isnot well known, except that cell migration and the actin cytoskeletonplay a prominent role. Microtubules are cytoskeletal components essential for cell migration. The present investigation determines the relationship between polyamines, the distribution of microtubules, and gastric healing in mucosa damaged with hypertonic NaCl solution. Rats were fasted for 22 h and then given 1.0 mi of 3.4 M NaCl intragastrically. Animals were killed 1, 2, 4, 8, and 10 h after 3.4 ill NaCl. The oxyntic gland mucosa was removed, and tubulin was visualized by immunofluorescence. Microtubule density was increased around and below the damaged mucosa in the upper one-third of the glandular epithelium at 2 and4 h and returned to near control levels by 10 h. In rats damaged with3.4 M NaCl and pretreated intraperitoneally with alpha-difluoromethylornithine (DFMO), a specific inhibitor of ODC, microtubule content wasreduced significantly at all time points after NaCl treatment. Addition of spermidine after pretreatment with DFMO and 3.4 M NaCl significantly prevented the effects of DFMO. Colchicine, a potent microtubule-disrupting drug, significantly delayed normal gastric mucosal healing with no effect on ODC activity. These data show that polyamines influence the distribution of microtubules during damage in vivo and indicatea partial mechanism for the dependency of mucosal healing on polyamines.

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Documento generato il 01/12/20 alle ore 13:39:56