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Titolo:
IN-SITU AMPLIFICATION OF THE EPSTEIN-BARR-VIRUS GENOME IN CELL-SUSPENSIONS
Autore:
FARES F; HABIB M; VERNIOL C; DROUET E; NIVELEAU A;
Indirizzi:
UNIV CLAUDE BERNARD,FAC MED,CTR COMMUN QUANTIMETRIE,8 AVE ROCKEFELLERF-69373 LYON 08 FRANCE UNIV CLAUDE BERNARD,FAC MED,CTR COMMUN QUANTIMETRIE F-69373 LYON 08 FRANCE UNIV JOSEPH FOURIER,FAC PHARM,LAB MICROBIOL & IMMUNOL,CNRS,UPRES A 5082 F-38706 LA TRONCHE FRANCE
Titolo Testata:
Journal of virological methods
fascicolo: 2, volume: 71, anno: 1998,
pagine: 211 - 218
SICI:
0166-0934(1998)71:2<211:IAOTEG>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; PCR-AMPLIFIED HIV-1; INSITU HYBRIDIZATION; PERIPHERAL-BLOOD; VIRAL-DNA; INTRACELLULAR AMPLIFICATION; INFECTIOUS-MONONUCLEOSIS; HUMAN PAPILLOMAVIRUS; HEALTHY-INDIVIDUALS; TISSUE-SECTIONS;
Keywords:
EPSTEIN-BARR VIRUS; IN SITU AMPLIFICATION; CELL SUSPENSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
F. Fares et al., "IN-SITU AMPLIFICATION OF THE EPSTEIN-BARR-VIRUS GENOME IN CELL-SUSPENSIONS", Journal of virological methods, 71(2), 1998, pp. 211-218

Abstract

Epstein-Barr virus (EBV) is distributed widely throughout the world. Apart from a association with two geographically-restricted malignancies (Burkitt's lymphoma and nasopharyngeal carcinoma), EBV is thought to be implicated in the etiology of B-cell lymphoma in immunocompromised individuals. In these patients, monitoring the viral load in serum can provide useful information on the timing of the instigation of antiviral therapy, i.e. as soon as a rise is detected. PCR technology, owing to its high sensitivity, is used frequently in such situations. In order to gain further insight into the nature of the peripheral blood cells carrying the viral genome on a cell-by-cell basis, an in situ amplification technique was developed as a model using two cell lines growing in suspension, with the aim of distinguishing between EBV-positive and EBV-negative cells. Preliminary experiments were undertaken subsequently on clinical samples from patients with infectious mononucleosis and patients with lymphoma indicating that this technique might beuseful clinically. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 00:25:01