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Titolo:
L-TYPE CALCIUM-CHANNEL EXPRESSION DEPENDS ON THE DIFFERENTIATED STATEOF VASCULAR SMOOTH-MUSCLE CELLS
Autore:
GOLLASCH M; HAASE H; RIED C; LINDSCHAU C; MORANO I; LUFT FC; HALLER H;
Indirizzi:
HUMBOLDT UNIV,MAX DELBRUCK CTR MOL MED,FRANZ VOLLARD CLIN,WILTBERG STR 50 D-13122 BERLIN GERMANY HUMBOLDT UNIV,MAX DELBRUCK CTR MOL MED,FRANZ VOLLARD CLIN D-13122 BERLIN GERMANY MAX DELBRUCK CTR MOL MED BERLIN GERMANY
Titolo Testata:
The FASEB journal
fascicolo: 7, volume: 12, anno: 1998,
pagine: 593 - 601
SICI:
0892-6638(1998)12:7<593:LCEDOT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; PROTEIN-KINASE-C; BETA-SUBUNIT; CA2+ CHANNELS; FUNCTIONAL EXPRESSION; RAT PHEOCHROMOCYTOMA; CARDIAC-MUSCLE; CURRENTS; HEART; LINE;
Keywords:
VOLTAGE-DEPENDENT CA2+ CHANNELS; DIHYDROPYRIDINES; ARTERIAL SMOOTH MUSCLE; DIFFERENTIATION; RETINOIC ACID; A7R5 CELLS; ATHEROSCLEROSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
M. Gollasch et al., "L-TYPE CALCIUM-CHANNEL EXPRESSION DEPENDS ON THE DIFFERENTIATED STATEOF VASCULAR SMOOTH-MUSCLE CELLS", The FASEB journal, 12(7), 1998, pp. 593-601

Abstract

Despite intensive interest in understanding the differentiation of vascular smooth muscle cells (VSMC), no information is available about differential regulation of ion channels in these cells. Since expression of the L-type Ca2+ channel can be influenced by differentiation in other cell types, we tested the hypothesis that the L-ype (C class) channel is a specific differentiation marker of VSMC and that expression of these channels depends on the state of cell differentiation. We used rat aortic (A7r5) VSMC, which express functional L-type Ca2+ channels, and induced dedifferentiation by cell culture in different media. Treatment with retinoic acid was used to redifferentiate the VSMC. We characterized the differentiated state of the cells by using immunohistochemistry and Western blot analysis for smooth muscle (SM) alpha-actin and SM-myosin heavy chain (MHC). The number of functional Ca2+ channels was significantly decreased in dedifferentiated VSMC and increasedupon differentiation with retinoic acid. Ca2+ channel function was assessed by whole-cell voltage clamp techniques. Using Western blot and dihydropyridine binding analysis, we found that the expression of the Ca2+ channel alpha(1) subunit, and to a lesser extent the beta(2) subunit, was directly correlated with the expression of SM alpha-actin andSM-MHC. We conclude that expression of L-type Ca2+ channel alpha(1) subunits, and thus a functional Ca2+ channel, is highly coordinated with expression of the SM-specific proteins required for specialized smooth muscle cell functions. Furthermore, our results demonstrate that the L-type Ca2+ channel is a novel marker for differentiation of VSMC. The data suggest that regulation of ion channel expression during differentiation may have physiological importance for normal smooth muscle function and may influence VSMC behavior under pathophysiological conditions.

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Documento generato il 05/12/20 alle ore 01:37:15