Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
XGRIP109 - A GAMMA-TUBULIN-ASSOCIATED PROTEIN WITH AN ESSENTIAL ROLE IN GAMMA-TUBULIN RING COMPLEX (GAMMA-TURC) ASSEMBLY AND CENTROSOME FUNCTION
Autore:
MARTIN OC; GUNAWARDANE RN; IWAMATSU A; ZHENG YX;
Indirizzi:
CARNEGIE INST WASHINGTON,DEPT EMBRYOL,115 W UNIV PKWY BALTIMORE MD 21210 CARNEGIE INST WASHINGTON,DEPT EMBRYOL BALTIMORE MD 21210 KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL YOKOHAMA KANAGAWA 236 JAPAN
Titolo Testata:
The Journal of cell biology
fascicolo: 3, volume: 141, anno: 1998,
pagine: 675 - 687
SICI:
0021-9525(1998)141:3<675:X-AGPW>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPINDLE POLE BODY; MICROTUBULE-ORGANIZING CENTER; IN-VITRO; SACCHAROMYCES-CEREVISIAE; ASPERGILLUS-NIDULANS; NUCLEATION; COMPONENT; TUB4P; IDENTIFICATION; ORGANIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
O.C. Martin et al., "XGRIP109 - A GAMMA-TUBULIN-ASSOCIATED PROTEIN WITH AN ESSENTIAL ROLE IN GAMMA-TUBULIN RING COMPLEX (GAMMA-TURC) ASSEMBLY AND CENTROSOME FUNCTION", The Journal of cell biology, 141(3), 1998, pp. 675-687

Abstract

Previous studies indicate that gamma tubulin ring complex (gamma TuRC) can nucleate microtubule assembly and may be important in centrosomeformation. gamma TuRC contains approximately eight subunits, which werefer to as Xenopus gamma ring proteins (Xgrips), in addition to gamma tubulin. We found that one gamma TuRC subunit, Xgrip109, is a highlyconserved protein, with homologues present in yeast, rice, flies, zebrafish, mice, and humans. The yeast Xgrip109 homologue, Spc98, is a spindle-pole body component that interacts with gamma tubulin. In vertebrates, Xgrip109 identifies two families of related proteins. Xgrip109 and Spc98 have more homology to one family than the other. We show that Xgrip109 is a centrosomal protein that directly interacts with gammatubulin. We have developed a complementation assay for centrosome formation using demembranated Xenopus sperm and Xenopus egg extract. Using this assay, we show that Xgrip109 is necessary for the reassembly ofsalt-disrupted gamma TuRC and for the recruitment of gamma tubulin tothe centrosome. Xgrip109, therefore, is essential for the formation of a functional centrosome.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 18:22:53