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Titolo:
THE RELATED ADHESION FOCAL TYROSINE KINASE (RAFTK) IS TYROSINE-PHOSPHORYLATED AND PARTICIPATES IN COLONY-STIMULATING FACTOR-I MACROPHAGE-COLONY-STIMULATING FACTOR SIGNALING IN MONOCYTE-MACROPHAGES
Autore:
HATCH WC; GANJU RK; HIREGOWDARA D; AVRAHAM S; GROOPMAN JE;
Indirizzi:
HARVARD UNIV,SCH MED,BETH ISRAEL DEACONESS MED CTR,HARVARD INST MED,DIV EXPT MED BOSTON MA 02115 HARVARD UNIV,SCH MED,BETH ISRAEL DEACONESS MED CTR,HARVARD INST MED,DIV EXPT MED BOSTON MA 02115 HARVARD UNIV,SCH MED,BETH ISRAEL DEACONESS MED CTR,HARVARD INST MED,DIV HEMATOL ONCOL BOSTON MA 02115
Titolo Testata:
Blood
fascicolo: 10, volume: 91, anno: 1998,
pagine: 3967 - 3973
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURED HUMAN MACROPHAGES; PHOSPHATIDYLINOSITOL 3-KINASE; HEMATOPOIETIC-CELLS; C-CBL; PROTEIN; ACTIVATION; ASSOCIATION; EXPRESSION; INTEGRINS; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
W.C. Hatch et al., "THE RELATED ADHESION FOCAL TYROSINE KINASE (RAFTK) IS TYROSINE-PHOSPHORYLATED AND PARTICIPATES IN COLONY-STIMULATING FACTOR-I MACROPHAGE-COLONY-STIMULATING FACTOR SIGNALING IN MONOCYTE-MACROPHAGES", Blood, 91(10), 1998, pp. 3967-3973

Abstract

RAFTK, a novel nonreceptor protein kinase, has been shown to be involved in focal adhesion signal transduction pathways in neuronal PC12 cells, megakaryocytes, platelets, and T cells. Because focal adhesions may modulate cytoskeletal functions and thereby alter phagocytosis, cell migration, and adhesion in monocyte-macrophages, we investigated therole of RAFTK signaling in these cells. RAFTK was abundantly expressed in THP1 monocytic cells as well as in primary alveolar and peripheral blood-derived macrophages. Colony-stimulating factor-1 (CSF-l)/macrophage colony-stimulating factor (M-CSF) stimulation of THP1 cells increased the tyrosine phosphorylation of RAFTK; similar increases in phosphorylation were also detected after lipopolysaccharide stimulation. RAFTK was phosphorylated with similar kinetics in THP1 cells and peripheral blood-derived macrophages. Immunoprecipitation analysis showed associations between RAFTK and the signaling molecule phosphatidylinositol-3 (PI-3) kinase, PI-3 kinase enzyme activity also coprecipitated with the RAFTK antibody, further confirming this association, The CSF-1/M-CSF receptor c-fms and RAFTK appeared to associate in response to CSF-1/M-CSF treatment of THP1 cells. Inhibition of RAFTK by a dominant-negative kinase mutant reduced CSF-1/M-CSF-induced MAPK activity. Thesedata indicate that RAFTK participates in signal transduction pathwaysmediated by CSF-1/M-CSF, a cytokine that regulates monocyte-macrophage growth and function. (C) 1998 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 18:00:56