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Titolo:
IMMUNOCYTOCHEMICAL LOCALIZATION OF AUXIN-BINDING PROTEINS IN COLEOPTILES AND EMBRYOS OF ZEA-MAYS L
Autore:
BRONSEMA FBF; VANOOSTVEEN WJF; VANLAMMEREN AAM;
Indirizzi:
AGR UNIV WAGENINGEN,GRAD SCH EXPT PLANT SCI,LAB PLANT CYTOL & MORPHOL,ARBORETUMLAAN 4 NL-6703 BD WAGENINGEN NETHERLANDS
Titolo Testata:
Protoplasma
fascicolo: 1-2, volume: 202, anno: 1998,
pagine: 65 - 75
SICI:
0033-183X(1998)202:1-2<65:ILOAPI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA-MEMBRANE; IMMUNOLOGICAL METHODS; CORN COLEOPTILES; HORMONE AUXIN; PLANT-CELL; MAIZE; PROTOPLASTS; ANTIBODIES; SIGNAL; PURIFICATION;
Keywords:
AUXIN-BINDING PROTEIN ABP-1; IMMUNOELECTRON MICROSCOPY; IMMUNOLOCALIZATION; ZEA MAYS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
F.B.F. Bronsema et al., "IMMUNOCYTOCHEMICAL LOCALIZATION OF AUXIN-BINDING PROTEINS IN COLEOPTILES AND EMBRYOS OF ZEA-MAYS L", Protoplasma, 202(1-2), 1998, pp. 65-75

Abstract

The auxin-binding protein ABP-1 was localised immunocytochemically incoleoptiles and immature embryos of Zea mays. Two primary polyclonal antibodies raised against ABP-1 and secondary antibodies were either labelled with FITC or 10 nm gold particles for light microscopy, and with 10 nm gold particles for transmission electron microscopy. Light microscopy revealed that ABP-1 was localised in the epidermal cells of etiolated maize coleoptiles, in subepidermal parenchymatic mesophyll cells of the coleoptile and in the companion cells of the vascular bundles. Most labelling was found in the cytoplasm, less in nuclei and vacucles and cell walls appeared negative. The region of the plasma membrane exhibited prominent labelling. Embryos showed low labelling throughout their tissues just after excision, but after culture for 7 days intensive labelling was found in the epidermis of the scutellum. Quantitative electron microscopy confirmed that ABP-1 was present in the cytoplasm of epidermal, mesophyll, and companion cells of coleoptiles. Gold particles were neither found in cell walls nor in the cuticle. Areaswith ER and dictyosomes within epidermal and mesophyll cells of coleoptiles had a denser labelling with gold particles than elsewhere. Labelling at the plasma membrane, being he site where the auxin binds to the ABP, was observed at low levels in all cells examined, which is dueto the method applied. Epidermal cells of embryos cultured for 5 daysexhibited high levels of gold particles in ER and nuclei, and lower levels in the cytoplasm. The distribution is only partly in accordance with the model in which ABP is thought to cycle through the plant cellfrom the ER via the Golgi system towards the plasma membrane.

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Documento generato il 27/11/20 alle ore 04:13:49