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Titolo:
SYNERGISTIC EFFECT OF TYPE-II PHOSPHOLIPASE A(2) AND PLATELET-ACTIVATING-FACTOR ON MAC-1 SURFACE EXPRESSION AND EXOCYTOSIS OF GELATINASE GRANULES IN HUMAN NEUTROPHILS - EVIDENCE FOR THE 5-LIPOXYGENASE-DEPENDENT MECHANISM
Autore:
TAKASAKI J; KAWAUCHI Y; MASUHO Y;
Indirizzi:
YAMANOUCHI PHARMACEUT CO LTD,INST DRUG DISCOVERY RES,21 MIYUKIGAOKA TSUKUBA IBARAKI 305 JAPAN
Titolo Testata:
The Journal of immunology
fascicolo: 10, volume: 160, anno: 1998,
pagine: 5066 - 5072
SICI:
0022-1767(1998)160:10<5066:SEOTPA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN POLYMORPHONUCLEAR LEUKOCYTES; ENDOTHELIAL-CELLS; FACTOR ACETYLHYDROLASE; CALCIUM; ADHESION; ACID; MOBILIZATION; ARTHRITIS; RELEASE; STORES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
J. Takasaki et al., "SYNERGISTIC EFFECT OF TYPE-II PHOSPHOLIPASE A(2) AND PLATELET-ACTIVATING-FACTOR ON MAC-1 SURFACE EXPRESSION AND EXOCYTOSIS OF GELATINASE GRANULES IN HUMAN NEUTROPHILS - EVIDENCE FOR THE 5-LIPOXYGENASE-DEPENDENT MECHANISM", The Journal of immunology, 160(10), 1998, pp. 5066-5072

Abstract

Stimulation of human neutrophils with inflammatory mediators such as TNF-alpha or platelet-activating factor (PAF) induces translocation ofadhesion molecule Mac-1 (CD11b/CD18) from secretory vesicles to the plasma membrane, Type II phospholipase A(2) (PLA(2)-II) also induces translocation of Mac-1 from secretory vesicles. However, there are more Mac-1 molecules in gelatinase granules and specific granules than in secretory vesicles. Therefore, different combinations of PLA(2)-II and other mediators were examined for their ability to induce gelatinase granules and specific granules to induce Mac-1 surface expression. The combination of PLA(2)-II and PAF synergistically increased Mac-1 surface expression, and the effect was greater than the combinations of PLA(2)-II with TNF-alpha, IL-8, or FMLP, Additionally, the combination ofPLA(2)-II and PAF induced exocytosis of both secretory vesicles and gelatinase granules, which did not occur with either PLA(2)-II alone orPAF alone. The induction was accompanied by marked production of leukotriene B-4. AA861, an inhibitor of 5-lipoxygenase, did not inhibit exocytosis of secretory vesicles but did inhibit exocytosis of gelatinase granules and decrease Mac-1 surface expression. It was also found that Ca2+ influx is essential for 5-lipoxygenase activation, because Ni2, which blocks the influx of extracellular Ca2+, inhibited the production of leukotriene B-4. These results suggest that stimulation by thecombination of PLA(2)-II and PAF, unlike stimulation by each mediatoralone, causes exocytosis of gelatinase granules via the 5-lipoxygenase pathway, resulting in a synergistic increase in neutrophil Mac-1 surface expression during inflammatory processes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 07:54:14