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Titolo:
ESTROGEN-INDUCED ALTERATION OF MU-OPIOID RECEPTOR IMMUNOREACTIVITY INTHE MEDIAL PREOPTIC NUCLEUS AND MEDIAL AMYGDALA
Autore:
ECKERSELL CB; POPPER P; MICEVYCH PE;
Indirizzi:
UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROBIOL LOS ANGELES CA 90095 UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROBIOL LOS ANGELES CA 90095 UNIV CALIF LOS ANGELES,BRAIN RES INST,NEUROENDOCRINOL LAB LOS ANGELESCA 90095
Titolo Testata:
The Journal of neuroscience
fascicolo: 10, volume: 18, anno: 1998,
pagine: 3967 - 3976
SICI:
0270-6474(1998)18:10<3967:EAOMRI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-COUPLED RECEPTORS; MESSENGER-RNA LEVELS; OPIATE RECEPTOR; LUTEINIZING-HORMONE; BETA-ENDORPHIN; GONADOTROPIN-SECRETION; FEMALE RAT; INTRACELLULAR TRAFFICKING; VENTROMEDIAL HYPOTHALAMUS; BEHAVIORAL SPECIFICITY;
Keywords:
RECEPTOR INTERNALIZATION; NEUROCHEMISTRY OF REPRODUCTION; STEROID HORMONES; OPIOID PEPTIDES; G-PROTEIN-COUPLED RECEPTORS; HYPOTHALAMUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
66
Recensione:
Indirizzi per estratti:
Citazione:
C.B. Eckersell et al., "ESTROGEN-INDUCED ALTERATION OF MU-OPIOID RECEPTOR IMMUNOREACTIVITY INTHE MEDIAL PREOPTIC NUCLEUS AND MEDIAL AMYGDALA", The Journal of neuroscience, 18(10), 1998, pp. 3967-3976

Abstract

The mu-opioid receptor (mu-OR), like most G-protein-coupled receptors, is rapidly internalized after agonist binding. Although opioid peptides induce internalization in vivo, there are no studies that demonstrate mu-OR internalization in response to natural stimuli. In this study, we used laser-scanning microscopy to demonstrate that estrogen treatment induces the translocation of mu-OR immunoreactivity (mu-ORi) from the membrane to an internal location in steroid-sensitive cell groups of the limbic system and hypothalamus. Estrogen-induced internalization was prevented by the opioid antagonist naltrexone, suggesting thattranslocation was largely dependent on release of endogenous agonists. Estrogen treatment also altered the pattern of mu-ORi at the bright-field light microscopic level. In the absence of stimulation, the majority of immunoreactivity is diffuse, with few definable mu-OR+ cell bodies or processes. After stimulation, the density of distinct processes filled with mu-ORi was significantly increased. We interpreted the increase in the number of mu-OR+ processes as indicating increased levels of internalization. Using this increase in the density of mu-OR+ fibers, we showed that treatment of ovariectomized rats with estradiol benzoate induced a rapid and reversible increase in the number of fibers. Significant internalization was noted within 30 min and lasted for >24 hr after estrogen treatment in the medial preoptic nucleus, the principal part of the bed nucleus, and the posterodorsal medial amygdala. Naltrexone prevented the increase of mu-OR+ processes. These data imply that estrogen treatment stimulates the release of endogenous opioids that activate mu-OR in the limbic system ana hypothalamus providinga ''neurochemical signature'' of steroid activation of these circuits.

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Documento generato il 05/04/20 alle ore 22:57:02