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Titolo:
ENHANCED FORMATION OF ROOTS AND SUBSEQUENT PROMOTION OF GROWTH OF SHOOTS ON CRYOPRESERVED NODAL SEGMENTS OF ASPARAGUS-OFFICINALIS L
Autore:
SUZUKI T; KANEKO M; HARADA T; YAKUWA T;
Indirizzi:
HOKKAIDO UNIV,FAC AGR,DEPT HORT SAPPORO HOKKAIDO 060 JAPAN
Titolo Testata:
Cryobiology
fascicolo: 3, volume: 36, anno: 1998,
pagine: 194 - 205
SICI:
0011-2240(1998)36:3<194:EFORAS>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
TISSUE FIXATION METHOD; PHOENIX-DACTYLIFERA L; LIQUID-NITROGEN; ENCAPSULATION-DEHYDRATION; MICROWAVE IRRADIATION; FREEZE PRESERVATION; MERISTEMS; SURVIVAL; INVITRO; PLANTLETS;
Keywords:
ASPARAGUS OFFICINALIS L.; CELL SURVIVAL; CRYOPRESERVATION; DOME-SHAPED APICAL MERISTEM; FREEZE-THAW; ORGANOGENESIS; ULTRASTRUCTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
T. Suzuki et al., "ENHANCED FORMATION OF ROOTS AND SUBSEQUENT PROMOTION OF GROWTH OF SHOOTS ON CRYOPRESERVED NODAL SEGMENTS OF ASPARAGUS-OFFICINALIS L", Cryobiology, 36(3), 1998, pp. 194-205

Abstract

This study was designed to investigate the effects of cryopreservation on the survival, organogenesis, and growth of plants regenerated from nodal segments of asparagus (Asparagus officinalis L.) that had beencut from cultures in vitro. The addition of dimethylsulfoxide (Me2SO)to the freezing solution at 8-16% (v/v) with or without a sugar (glucose, sorbitol, or sucrose) was effective for successful cryopreservation by a slow prefreezing method. Frequencies of root formation (average, 59.3%) from cryopreserved and surviving nodal segments were significantly higher (P < 0.005) than those (average, 13.9%) from nodal segments that had only been treated with freezing solution supplemented with 12% (v/v) Me2SO and various sugars without freeze-thawing. The increased frequency of root formation from cryopreserved nodal segments appears to have been induced by the freeze-thaw step of the cryopreservation procedure. Numbers and lengths of shoots derived from cryopreserved nodal segments were initially lower but were subsequently higher, after 60-90 days of culture, than those of shoots derived From nodal segments without freeze-thawing. The promotion of growth of shoots from cryopreserved nodal segments seemed to have been due to the increased percentage of root formation. Histological observations revealed that only dome-shaped meristematic tissue and a few cells of cladophyll primordia survived in cryopreserved nodal segments that had been cultured for 5 days after thawing. Many mitochondria and well developed rER were observed in these cells. Disorganization and/or physiological changes might have occurred in thr surviving tissues and/or cells of the cryopreserved nodal segments that were responsible for the subsequent increased formation of roots, (C) 1998 Academic Press.

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Documento generato il 29/03/20 alle ore 18:17:58