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Titolo:
AN ENZYMATIC METHOD FOR THE DETERMINATION OF FREE FATTY-ACIDS IN SERUM PLASMA/
Autore:
KIZILTUNC A; AKCAY F;
Indirizzi:
ATATURK UNIV,SCH MED,DEPT BIOCHEM TR-25240 ERZURUM TURKEY
Titolo Testata:
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
fascicolo: 2, volume: 36, anno: 1998,
pagine: 83 - 86
SICI:
1434-6621(1998)36:2<83:AEMFTD>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERUM;
Keywords:
FREE FATTY ACIDS; SERUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
9
Recensione:
Indirizzi per estratti:
Citazione:
A. Kiziltunc e F. Akcay, "AN ENZYMATIC METHOD FOR THE DETERMINATION OF FREE FATTY-ACIDS IN SERUM PLASMA/", CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 36(2), 1998, pp. 83-86

Abstract

Estimation of serum free fatty acids (FFA) in serum based on the formation of inorganic phosphate has been simplified by eliminating complex stages. The principle of the present method is based on breakdown ofpyrophosphate, formed by thioesterification of free fatty acids with ATP and CoA with the aid of acyl-CoA synthetase (EC 6.2.1.3) to inorganic phosphate. This is measured using the reaction with molybdate. Thereaction equations are as follows: [GRAPHICS] The recovery of free fatty acids was 96%. The interferences of citrate, phosphatidylserine, succinate, ascorbic acid and lecithin were between 0.5 and 2%. The correlation between the new enzymatic and the classic enzymatic method was0.966. The lower detection limit was 0.018 mmol/l. The method was linear between 0.02 and 2.0 mmol/l. The within-assay and between-assay imprecision (CV) of control sera was 5.5% and 8%, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 13:50:33