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Titolo:
RESTORATION OF LECTIN ACTIVITY TO A NONGLYCOSYLATED RICIN B-CHAIN MUTANT BY THE INTRODUCTION OF A NOVEL N-GLYCOSYLATION SITE
Autore:
ZHAN JB; DESOUSA M; CHADDOCK JA; ROBERTS LM; LORD JM;
Indirizzi:
UNIV WARWICK,DEPT BIOL SCI COVENTRY CV4 7AL W MIDLANDS ENGLAND UNIV WARWICK,DEPT BIOL SCI COVENTRY CV4 7AL W MIDLANDS ENGLAND
Titolo Testata:
FEBS letters
fascicolo: 3, volume: 407, anno: 1997,
pagine: 271 - 274
SICI:
0014-5793(1997)407:3<271:ROLATA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GALACTOSE-BINDING ABILITY; COMMUNIS AGGLUTININ; MUTATIONAL ANALYSIS; ESCHERICHIA-COLI; EXPRESSION; SYSTEM;
Keywords:
RICIN B CHAIN; LECTIN ACTIVITY; N-GLYCOSYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
J.B. Zhan et al., "RESTORATION OF LECTIN ACTIVITY TO A NONGLYCOSYLATED RICIN B-CHAIN MUTANT BY THE INTRODUCTION OF A NOVEL N-GLYCOSYLATION SITE", FEBS letters, 407(3), 1997, pp. 271-274

Abstract

Ricin B chain (RTB) is an N-glycosylated, galactose-specific lectin, Removal of the two native N-glycosylation sites at Asn(95) and Asn(135) by site-directed mutagenesis generated a recombinant protein devoid of lectin activity, Two novel N-glycosylation sites were introduced into RTB at Asn(42) and Asn(123), either singly or in combination, Microinjection of pre-RTB transcripts into Xenopus oocytes showed that these novel sites became glycosylated in vivo, The single oligosaccharide site chain at Asn(42) restored lectin activity to RTB, whereas glycosylation at Asn(123) or simultaneous glycosylation at Asn(42) and Asn(123) failed to do so. (C) 1997 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 18:31:18