Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
OSTEOGENIC PROTEIN (OP-1, BMP-7) STIMULATES CARTILAGE DIFFERENTIATIONOF HUMAN AND GOAT PERICHONDRIUM TISSUE IN-VITRO
Autore:
KLEINNULEND J; LOUWERSE RT; HEYLIGERS IC; WUISMAN PIJM; SEMEINS CM; GOEI SW; BURGER EH;
Indirizzi:
FREE UNIV AMSTERDAM,ACTA,DEPT ORAL CELL BIOL,VAN DER BOECHORSTSTR 7 NL-1081 BT AMSTERDAM NETHERLANDS FREE UNIV AMSTERDAM,ACAD HOSP,DEPT ORTHOPAED NL-1081 BT AMSTERDAM NETHERLANDS
Titolo Testata:
Journal of biomedical materials research
fascicolo: 4, volume: 40, anno: 1998,
pagine: 614 - 620
SICI:
0021-9304(1998)40:4<614:OP(BSC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARTICULAR-CARTILAGE; PROLIFERATION; CELLS; BETA;
Keywords:
CARTILAGE; GROWTH FACTORS; TISSUE ENGINEERING; OSTEOGENIC PROTEIN-1; CARTILAGE REPAIR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
J. Kleinnulend et al., "OSTEOGENIC PROTEIN (OP-1, BMP-7) STIMULATES CARTILAGE DIFFERENTIATIONOF HUMAN AND GOAT PERICHONDRIUM TISSUE IN-VITRO", Journal of biomedical materials research, 40(4), 1998, pp. 614-620

Abstract

The objective of this study was to examine in vitro the influence of recombinant human osteogenic protein-1 [rhOP-1, or bone morphogenetic protein-7 (BMP-7)] on cartilage formation by human and goat perichondrium tissue containing progenitor cells with chondrogenic potential. Fragments of outer ear perichondrium tissue were embedded in clotting autologous blood to which rhOP-1 had been added or not added (controls),and the resulting explant was cultured for 3 weeks without further addition of rhOP-1. Cartilage formation was monitored biochemically by measuring [S-35]-sulphate incorporation into proteoglycans and histologically by monitoring the presence of metachromatic matrix with cells in nests. The presence of rhOP-1 in the explant at the beginning of culture stimulated [S-35]- sulphate incorporation into proteoglycans in adose-dependent manner after 3 weeks of culture. Maximal stimulation was reached at 40 mu g/mL, (human explants: +148%; goat explants: +116%). Histology revealed that explants treated with 20-200 mu g/mL of rhOP-1, but not untreated control explants, contained areas of metachromatic-staining matrix with chondrocytes in cell nests. It was concluded that rhOP-1 stimulates differentiation of cartilage from perichondriumtissue. The direct actions of rhOP-1 on perichondrium cells in the stimulation of chondrocytic differentiation and production of cartilage matrix in vitro provides a cellular mechanism for the induction of cartilage formation by rhOP-1 in vitro. Thus rhOP-1 may promote early steps in the cascade of events leading to cartilage formation and could prove to be an interesting factor in the regeneration of cartilage in articular cartilage defects. (C) 1998 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 09:37:59