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Titolo:
IDENTIFICATION OF AN ENDONUCLEASE SECRETED BY HUMAN B-LYMPHOBLASTIC IM9 CELLS
Autore:
KWON HJ; KIM DS;
Indirizzi:
YONSEI UNIV,DEPT BIOCHEM,COLL SCI SEOUL 120749 SOUTH KOREA YONSEI UNIV,DEPT BIOCHEM,COLL SCI SEOUL 120749 SOUTH KOREA YONSEI UNIV,COLL SCI,DEPT BIOCHEM SEOUL 120749 SOUTH KOREA
Titolo Testata:
International journal of biochemistry & cell biology
fascicolo: 2, volume: 30, anno: 1998,
pagine: 217 - 223
SICI:
1357-2725(1998)30:2<217:IOAESB>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
APOPTOTIC RAT THYMOCYTES; PANCREATIC DNASE-I; DEOXYRIBONUCLEASE-I; DEPENDENT ENDONUCLEASE; BODY-FLUIDS; HUMAN-SERUM; PURIFICATION; URINE; MULTIPLICITY; DEGRADATION;
Keywords:
MG2+-DEPENDENT ENDONUCLEASE; DNA-NATIVE-POLYACRYLAMIDE GEL ELECTROPHORESIS; IM9 CELLS; SECRETION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
H.J. Kwon e D.S. Kim, "IDENTIFICATION OF AN ENDONUCLEASE SECRETED BY HUMAN B-LYMPHOBLASTIC IM9 CELLS", International journal of biochemistry & cell biology, 30(2), 1998, pp. 217-223

Abstract

We have identified a Mg2+-dependent endonuclease from IM9 cell lysates and culture medium using DNA-native-polyacrylamide gel electrophoresis (DNA-native-PAGE) nuclease assay system. This particular endonuclease activity was not detectable in conventional DNA-SDS-polyacrylamide gel electrophoresis assay system which is similar to the method originally described by Rosenthal and Lacks (A.L. Rosenthal and S.A. Lacks, Anal. Biochem. 80 (1977) 76-90). Experimental results clearly demonstrated that the endonuclease activity was not derived from the fetal calf serum in which the cells were grown, but synthesized in the cell andsecreted into the culture medium by IM9 cells. Biosynthesis and subsequent release of the endonuclease into the culture medium were significantly decreased by pretreatment of the cells with actinomycin D. Using supercoiled plasmid DNA as a substrate, the endonuclease activity was determined with the enzyme isolated from the cell culture medium by native-PAGE etectroelution. The endonuclease, with Mg2+ alone, was able to catalyze the conversion of the plasmid into linear DNA followed by further degradation. This is the first report demonstrating that a distinct Mg2+-dependent endonuclease is secreted by a human immune cellline. (C) 1998 Elsevier Science Ltd. All rights reserved.

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Documento generato il 05/07/20 alle ore 16:09:48