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Titolo:
CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION ANALYSIS OF GINGIPAIN R2 FROM PORPHYROMONAS-GINGIVALIS IN COMPLEX WITH H-D-PHE-PHE-ARG-CHLOROMETHYLKETONE
Autore:
BANBULA A; POTEMPA J; TRAVIS J; BODE W; MEDRANO FJ;
Indirizzi:
CSIC,CTR INVEST BIOL,DEPT MOL MICROBIOL,VELAZQUEZ 144 E-28006 MADRID SPAIN JAGIELLONIAN UNIV,INST MOL BIOL PL-31120 KRAKOW POLAND UNIV GEORGIA,DEPT BIOCHEM ATHENS GA 30602 MAX PLANCK INST BIOCHEM,ABT STRUKTURFORSCH D-82152 MARTINSRIED GERMANY
Titolo Testata:
Protein science
fascicolo: 5, volume: 7, anno: 1998,
pagine: 1259 - 1261
SICI:
0961-8368(1998)7:5<1259:CAPDAO>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEINASE; VIRULENCE; PERIODONTITIS; BACTEROIDES; W50;
Keywords:
CYSTEINE PROTEINASE; GINGIPAIN R2; GINGIPAINS; PORPHYROMONAS GINGIVALIS; PROTEIN CRYSTALLIZATION; X-RAY DIFFRACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
A. Banbula et al., "CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION ANALYSIS OF GINGIPAIN R2 FROM PORPHYROMONAS-GINGIVALIS IN COMPLEX WITH H-D-PHE-PHE-ARG-CHLOROMETHYLKETONE", Protein science, 7(5), 1998, pp. 1259-1261

Abstract

Gingipain R2 is a 50 kDa proteinase from the oral pathogenic bacterium Porphyromonas gingivalis. This proteinase, which displays no significant sequence homology to any protein previously analyzed by X-ray crystallography, has been crystallized using the vapor diffusion method. Two different crystal forms were obtained from a solution containing polyethylene glycol (MW 8,000) (space group P2(1)2(1)2(1)) or magnesiumsulfate (space group R3) as precipitating agent. Complete diffractiondata sets have been collected up to 2.0 and 2.9 Angstrom resolution, respectively. Cell dimensions are a = 51.9 Angstrom, b = 79.9 Angstrom, and c = 99.6 Angstrom (P2(1)2(1)2(1)), and a = b = 176.6 Angstrom, and c = 143.4 Angstrom (R3). Considerations of the possible values of V-m accounts for the presence of one monomer per asymmetric unit in thecase of the orthorhombic crystal form, whereas the rhombohedral crystal form, together with the analysis of the self-rotation function, could accommodate a tetramer in the asymmetric unit.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 16:51:28