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Titolo:
EFFECT OF COLD AND WARM ISCHEMIA ON DRUG-METABOLISM IN ISOLATED HEPATOCYTES AND SLICES FROM HUMAN AND MONKEY LIVER
Autore:
OLINGA P; MEREMA MT; HOF IH; DEJAGER MH; DEJONG KP; SLOOFF MJH; MEIJER DKF; GROOTHUIS GMM;
Indirizzi:
UNIV GRONINGEN,CTR PHARM,DEPT PHARMACOKINET & DRUG DELIVERY,GRONINGENINST DRUG STUDIES NL-9713 AV GRONINGEN NETHERLANDS UNIV GRONINGEN HOSP,DEPT SURG,DIV HEPATOBILIARY SURG & LIVER TRANSPLANTAT NL-9713 EZ GRONINGEN NETHERLANDS
Titolo Testata:
Xenobiotica
fascicolo: 4, volume: 28, anno: 1998,
pagine: 349 - 360
SICI:
0049-8254(1998)28:4<349:EOCAWI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
OF-WISCONSIN SOLUTION; RAT HEPATOCYTES; PHASE-I; CRYOPRESERVATION; BIOTRANSFORMATION; 7-ETHOXYCOUMARIN; PRESERVATION; MICROSOMES; VIABILITY; CULTURES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
P. Olinga et al., "EFFECT OF COLD AND WARM ISCHEMIA ON DRUG-METABOLISM IN ISOLATED HEPATOCYTES AND SLICES FROM HUMAN AND MONKEY LIVER", Xenobiotica, 28(4), 1998, pp. 349-360

Abstract

1. The influence of short-term cold storage in University of Wisconsin organ preservation solution (UW) on the ability to metabolize lidocaine, testosterone and 7-ethoxycoumarin in isolated human and cynomolgus monkey (Macaca fascicularis) hepatocytes and liver slices has been investigated. 2. The human liver tissue was obtained from two differentsources, i.e. healthy liver tissue from patients undergoing partial hepatectomy because of metastases of colorectal carcinoma (PH livers) and donor tissue remaining as surgical waste after reduced size or split liver transplantation (Tx livers). Tx livers were perfused in situ with ice-cold UW avoiding warm ischaemia. This in contrast with PH livers, where the operation caused warm ischaemia for 5-90 min. 3. Liver slices and hepatocytes from cynomolgus monkey liver showed comparable metabolic rates for the substrates tested, indicating that all hepatocytes in the slice are participating in the biotransformation of the substrates. These monkey liver preparations can be stored up to 18 h withonly a slight loss of their metabolic capacity. 4. Liver slices and isolated hepatocytes from the Tx livers as well as isolated cells from the PH livers could also be stored up to Is h without losing metaboliccapacity. However, for liver slices prepared from PH livers cold storage is not recommended, because metabolic function was reduced by approximately 40 % after 18 h.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 11:49:22