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Titolo:
REGENERATION OF POPULUS-NIGRA TRANSGENIC PLANTS EXPRESSING A KUNITZ PROTEINASE-INHIBITOR (KTI3) GENE
Autore:
CONFALONIERI M; ALLEGRO G; BALESTRAZZI A; FOGHER C; DELLEDONNE M;
Indirizzi:
POPLAR RES INST,VIA DI FRASSINETO 35 I-15033 CASALE MONFERRATO AL ITALY DEPT GENET & MICROBIOL A BUZZATI TRAVERSO I-27100 PAVIA ITALY UNIV CATTOLICA S CUORE,INST PLANT GENET I-29100 PIACENZO ITALY
Titolo Testata:
Molecular breeding
fascicolo: 2, volume: 4, anno: 1998,
pagine: 137 - 145
SICI:
1380-3743(1998)4:2<137:ROPTPE>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEASE INHIBITORS; SPODOPTERA-EXIGUA; TRYPSIN; MIDGUT; TRANSFORMATION; RESISTANCE; INSECTS; GROWTH; PURIFICATION; DIETARY;
Keywords:
AGROBACTERIUM TUMEFACIENS; INSECT PEST RESISTANCE; KUNITZ TRYPSIN PROTEINASE INHIBITOR; POPULUS NIGRA L.; TRANSGENIC PLANTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
M. Confalonieri et al., "REGENERATION OF POPULUS-NIGRA TRANSGENIC PLANTS EXPRESSING A KUNITZ PROTEINASE-INHIBITOR (KTI3) GENE", Molecular breeding, 4(2), 1998, pp. 137-145

Abstract

Transgenic poplar (Populus nigra, cv. Jean Pourtet) plants were recovered as a result of Agrobacterium tumefaciens-mediated transformation performed with EHA105 pBI-KUN strain. Plasmid pBI-KUN contains a 650 bp insert derived from the soybean (Glycine max L. ) KTi3, gene, coding for a Kunitz trypsin proteinase inhibitor. A total of 58 independent transgenic lines were obtained from 200 co-cultivated leaf explants. Southern blot hybridization analysis demonstrated the presence of KTi3 gene in the poplar genome. Northern blot analysis of different kanamycin-resistant plantlets confirmed the accumulation of KTi3 mRNA and revealed different levels of expression. The trypsin inhibitory activity was determined in poplar transgenic tissues by means of specific assay. Moreover, the trypsin-like digestive proteinases of the polyphagous moth Lymantria dispar (Lepidoptera, Lymantriidae) and Clostera anastomosis (Lepidoptera, Notodontidae) were detected and inhibited in vitro by Kunitz proteinase inhibitor from selected transgenic plants. Two insect bioassays were performed on P. nigra transgenic plant lines, usinglarvae of the above mentioned insects. In both cases larval mortalityand growth as well as pupal weight were not significantly affected when the insects were fed on transgenic leaves and control leaves, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 19:20:41