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Titolo:
A POSITIVE EFFECT OF P21(CIP1 WAF1) IN THE COLONY FORMATION FROM MURINE MYELOID PROGENITOR CELLS AS ASSESSED BY RETROVIRAL-MEDIATED GENE-TRANSFER/
Autore:
BRAUN SE; MANTEL C; ROSENTHAL M; COOPER S; LIU L; ROBERTSON KA; HROMAS R; BROXMEYER HE;
Indirizzi:
INDIANA UNIV,SCH MED,WALTHER ONCOL CTR,1044 W WALNUT ST,ROOM 302 R4 INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,DEPT MICROBIOL IMMUNOL INDIANAPOLIS IN 46202 WALTHER CANC INST INDIANAPOLIS IN 46208 INDIANA UNIV,SCH MED,DEPT PEDIAT INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,WELLS CTR PEDIAT RES INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,DEPT MED HEMATOL ONCOL INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,DEPT BIOCHEM MOL BIOL INDIANAPOLIS IN 46202
Titolo Testata:
Blood cells, molecules, & diseases
fascicolo: 8, volume: 24, anno: 1998,
pagine: 138 - 148
SICI:
1079-9796(1998)24:8<138:APEOPW>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIN-DEPENDENT KINASES; ACTIVATED PROTEIN-KINASE; HEMATOPOIETIC STEM-CELLS; STIMULATING FACTOR; GROWTH-FACTOR; P21(WAF1/CIP1) EXPRESSION; P21; MICE; DIFFERENTIATION; INTERLEUKIN-3;
Keywords:
P2L(CIP1/WAF1); GENE TRANSFER; P21(CIP1/WAF1) KNOCKOUT MICE; HEMATOPOIETIC PROGENITOR CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
S.E. Braun et al., "A POSITIVE EFFECT OF P21(CIP1 WAF1) IN THE COLONY FORMATION FROM MURINE MYELOID PROGENITOR CELLS AS ASSESSED BY RETROVIRAL-MEDIATED GENE-TRANSFER/", Blood cells, molecules, & diseases, 24(8), 1998, pp. 138-148

Abstract

p21(cip1/waf1) is cyclin dependent kinase inhibitor, We have previously reported stimulation of p21(cip1/waf1) by steel factor and GM-CSF in a factor dependent cell Line and of p21(cip1/waf1) involvement in hematopoiesis in vivo in p21(cip1/waf1) gene knockout (-/-) mice. To further assess, a role for increased p21(cip1/waf1) in hematopoietic progenitor cells, we developed the retroviral vector L(p21(cip1))SN to transcriptionally regulate p21(cip1/waf1) from the Mo-MLV LTR, L(p21(cip1))SN and the control vector LXSN were used to transduce murine bone marrow progenitor cells from p21(cip1/waf1) (-/-) and littermate control(+/+) mice, as well as from other mouse strains, Hematopoietic colonyformation by transduced cells was assessed in semi-solid culture medium with multiple growth factors, Myeloid colony formation by bone marrow cells from p21(cip1/waf1) (-/-) mice was significantly lower than that by (+/+) mouse cells. Transduction of cells with LXSN had no effect on colony formation; however, (-/-) cells transduced with L(p21(cip1))SN formed significantly greater numbers of colonies than either LXSN-transduced (-/-) or (+/+) cells. Moreover, L(p21(cip1))SN-transduced (+/+) cells formed significantly more colonies than LXSN-transduced (+/+) cells. Increased cloning efficiency of progenitors from normal strains of mice induced by L(p21(cip1))SN compared to LXSN-transduced cells was seen whether unseparated or highly purified populations of Sca1(+)Lin(-) marrow cells were used. Gene transfer of L(p21(cip1))SN increased the size and number of cells per colony, as well as the number of colonies compared to LXSN gene transfer, No colonies grew from non-transduced, LXSN-, or L(p21(cip1))SN-transduced cells when no growth factors were added to the cultures. These results document the positive effect of p21(cip1/waf1) in the proliferation and/or differentiation of the murine myeloid progenitor cells that lead to colony formation. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:20:00