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Titolo:
INVOLVEMENT OF H-RAS IN ERYTHROID-DIFFERENTIATION OF TF1 AND HUMAN UMBILICAL-CORD BLOOD CD34(+++) CELLS
Autore:
GE Y; LI ZH; MARSHALL MS; BROXMEYER HE; LU L;
Indirizzi:
INDIANA UNIV,SCH MED,WALTHER ONCOL CTR,1044 W WALNUT ST,ROOM 302 INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,WALTHER ONCOL CTR INDIANAPOLIS IN 46202 WALTHER CANC INST INDIANAPOLIS IN 46208 INDIANA UNIV,SCH MED,DEPT MED HEMATOL ONCOL INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,DEPT BIOCHEM MOL BIOL INDIANAPOLIS IN 46202
Titolo Testata:
Blood cells, molecules, & diseases
fascicolo: 8, volume: 24, anno: 1998,
pagine: 124 - 136
SICI:
1079-9796(1998)24:8<124:IOHIEO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR GATA-1; KIRSTEN SARCOMA-VIRUSES; ERYTHROPOIETIN RECEPTOR; GENE; ONCOGENES; GROWTH; EXPRESSION; HARVEY; LINE; PROLIFERATION;
Keywords:
H-RAS; ERYTHROID DIFFERENTIATION; TF1 CELLS; CD34(+++) CELLS; CORD BLOOD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
Y. Ge et al., "INVOLVEMENT OF H-RAS IN ERYTHROID-DIFFERENTIATION OF TF1 AND HUMAN UMBILICAL-CORD BLOOD CD34(+++) CELLS", Blood cells, molecules, & diseases, 24(8), 1998, pp. 124-136

Abstract

To investigate the role of the ras gene in erythroid differentiation,a human erythroleukemic cell line, TF1, was transduced with a selectable retroviral vector carrying a mammalian wild type H-ras gene or a cytoplasmic dominant negative RAS1 gene. Transduction of TF1 cells withthe wild type H-ras gene resulted in changes of cell types and up-regulation of erythroid-specific gene expression similar to that seen in differentiating erythroid cells. The number of red blood cell containing colonies derived from TF1 cells transduced with wild type H-ras cDNA was significantly increased and the cells in the colonies were more hemoglobinized as estimated by a deeper red color compared to those colony cells from mock or dominant negative RAS1 gene transduced TF1 cells, suggesting increased erythroid differentiation of TF1 cells after transduction of wild type H-ras in vitro. The mRNA levels of beta- andgamma-, but not alpha-, globin genes were significantly higher in H-ras transduced TF1 cells than those in TF1 cells transduced with mock or dominant negative RAS1 gene. Moreover, a 4kb pre-mRNA of the Erythropoietin receptor (EpoR) was highly expressed only in H-ras transduced TF1 cells, Additionally, human umbilical cord blood (CB) CD34(+++) cells which are highly enriched for hematopoietic stem/progenitor cells were transduced with the same retroviral vectors to evaluate in normal primary cells the activities of H-ras in erythroid differentiation. Increased numbers of erythroid cell containing colonies (BFU-E and CFU-GEMM) were observed in CD34(+++) cells transduced with the H-ras cDNA, compared to that from mock transduced cells. These data suggest a possible role for ras in erythroid differentiation. (C) 1998 Academic Press.

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Documento generato il 03/04/20 alle ore 07:47:29