Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
LEUKOTRIENE D-4 AND CYSTINYL-BIS-GLYCINE METABOLISM IN MEMBRANE-BOUNDDIPEPTIDASE-DEFICIENT MICE
Autore:
HABIB GM; SHI ZZ; CUEVAS AA; GUO QX; MATZUK MM; LIEBERMAN MW;
Indirizzi:
BAYLOR COLL MED,DEPT PATHOL HOUSTON TX 77030 BAYLOR COLL MED,DEPT PATHOL HOUSTON TX 77030 BAYLOR COLL MED,DEPT CELL BIOL HOUSTON TX 77030 BAYLOR COLL MED,DEPT MOL & HUMAN GENET HOUSTON TX 77030
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 9, volume: 95, anno: 1998,
pagine: 4859 - 4863
SICI:
0027-8424(1998)95:9<4859:LDACMI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-GLUTAMYL-TRANSPEPTIDASE; BETA-LACTAMASE ACTIVITY; RENAL DIPEPTIDASE; CYSTEINYL LEUKOTRIENES; DEHYDROPEPTIDASE-I; ENHANCED SYNTHESIS; PURIFICATION; ASTHMA; INFLAMMATION; THIENAMYCIN;
Keywords:
GLUTATHIONE EICOSANOIDS; HOMOLOGOUS RECOMBINATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
G.M. Habib et al., "LEUKOTRIENE D-4 AND CYSTINYL-BIS-GLYCINE METABOLISM IN MEMBRANE-BOUNDDIPEPTIDASE-DEFICIENT MICE", Proceedings of the National Academy of Sciences of the United Statesof America, 95(9), 1998, pp. 4859-4863

Abstract

We have developed mice deficient in membrane-bound dipeptidase (MBD, EC 3.4.13.19), the enzyme believed to be responsible for the conversion of leukotriene D-4 (LTD4) to leukotriene E-4 (LTE4). The MBD mutation generated by us was demonstrated to be a null mutation by Northern blot analysis and the absence of beta-lactamase activity in lung, kidney, small intestine, and heart. MBD gene deletion had no effect on viability or fertility. The mutant mice retain partial ability to convert LTD4 to LTE4, ranging from 80-90% of the wild-type values in small intestine and liver to 16% in kidney and 40% in lung, heart, and pancreas. MBD is also believed to function consecutively after gamma-glutamyl transpeptidase to cleave cystinyl-bis-glycine (cys-bis-gly) generated from glutathione cleavage. Our data indicate that kidney homogenates from MBD-deficient mice retain similar to 40% of their ability to cleave cys-bis-gly, consistent with only modest elevations (3-5-fold) of cys-bis-gly in urine from MBD-deficient mice, These observations demonstrate that the conversion of LTD4 to LTE4 and the degradation of cys-bis-gly are catalyzed by at least two alternative pathways (one of whichis MBD) that complement each other to varying extents in different tissues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 00:43:45