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Titolo:
INCREASED EXPRESSION OF THE NOVEL MOLECULE OX-2 IS INVOLVED IN PROLONGATION OF MURINE RENAL-ALLOGRAFT SURVIVAL
Autore:
GORCZYNSKI RM; CHEN ZQ; FU XM; ZENG H;
Indirizzi:
UNIV TORONTO,TORONTO HOSP,TRANSPLANTAT RES GRP,DEPT SURG & IMMUNOL,TRANSPLANT RES DIV TORONTO ON M5G 2C4 CANADA
Titolo Testata:
Transplantation
fascicolo: 8, volume: 65, anno: 1998,
pagine: 1106 - 1114
SICI:
0041-1337(1998)65:8<1106:IEOTNM>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORTAL VENOUS IMMUNIZATION; ANTIGEN-PRESENTING CELLS; IN-VIVO; GAMMA-PRODUCTION; SKIN ALLOGRAFTS; B7-2 EXPRESSION; MESSENGER-RNA; IFN-GAMMA; T-CELLS; RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
R.M. Gorczynski et al., "INCREASED EXPRESSION OF THE NOVEL MOLECULE OX-2 IS INVOLVED IN PROLONGATION OF MURINE RENAL-ALLOGRAFT SURVIVAL", Transplantation, 65(8), 1998, pp. 1106-1114

Abstract

Background. Portal venous (p.v.) peritransplant immunization with dendritic cells from bone marrow cultures, along with cyclosporine (10 mg/kg), produces antigen-specific increased renal allograft survival compared with recipients receiving intravenous (i.v.) immunization. Increased survival is associated with altered cytokine production from recipient T cells restimulated with donor antigen. We used a suppressive subtractive hybridization approach to explore a role in the regulation of transplant rejection for other genes differentially expressed afterp.v. immunization. Methods. Subtractive hybridization was performed using tissue from p.v. and i.v. immunized mice and a novel polymerase chain reaction-based approach. A gene-bank search was used to identify the source of the differentially expressed cDNAs. One product, the mouse homologue of rat OX-2, was further analyzed using Western gels and FACS analysis of dendritic cells (NLDC145(+)) isolated from p.v.-immunized mice. Results. Eighty cDNA clones were obtained by suppressive subtractive hybridization. Differential expression was confirmed in Northern RNA blots. One clone showed sequence homology to a gene encoding a molecule on rat dendritic cells (MRC OX-2), with homology to genes encoding the costimulatory molecules CD80 (B7-1) and CD86 (B7-2). In p.v.-immunized mice, a monoclonal antibody to the rat OX-2 molecule identified, by Western blot analysis, increased expression of a molecule with molecular weight (43 kDa) analagous to rat MRC-OX-2; labels (by FACS analysis) indentified increased numbers of a population of cells staining with NLDC145; and blocks indentified increased graft survival. Conclusion. Our data suggest that OX-2 is functionally important in the increased graft survival seen in p.v.-immunized mice receiving renalallografts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 01:58:11