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Titolo:
SULFHYDRYL OXIDATION MODIFIES THE CALCIUM-DEPENDENCE OF RYANODINE-SENSITIVE CALCIUM CHANNELS OF EXCITABLE CELLS
Autore:
MARENGO JJ; HIDALGO C; BULL R;
Indirizzi:
UNIV CHILE,FAC MED,INST CIENCIAS BIOMED,PROGRAMA FISIOL & BIOFIS,CASILLA 70005,CORREO 7 SANTIAGO 7 CHILE UNIV CHILE,FAC MED,INST CIENCIAS BIOMED,PROGRAMA BIOL MOL & CELULAR SANTIAGO 7 CHILE CTR ESTUDIOS CIENT SANTIAGO SANTIAGO 9 CHILE
Titolo Testata:
Biophysical journal
fascicolo: 3, volume: 74, anno: 1998,
pagine: 1263 - 1277
SICI:
0006-3495(1998)74:3<1263:SOMTCO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUSCLE SARCOPLASMIC-RETICULUM; CA-2+ RELEASE CHANNEL; CHICKEN SKELETAL-MUSCLE; CARDIAC-MUSCLE; SINGLE-CHANNEL; FREE-RADICALS; CA2+ RELEASE; CA2+-RELEASE CHANNELS; MOLECULAR-CLONING; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
J.J. Marengo et al., "SULFHYDRYL OXIDATION MODIFIES THE CALCIUM-DEPENDENCE OF RYANODINE-SENSITIVE CALCIUM CHANNELS OF EXCITABLE CELLS", Biophysical journal, 74(3), 1998, pp. 1263-1277

Abstract

The calcium dependence of ryanodine-sensitive single calcium channelswas studied after fusing with planar lipid bilayers sarcoendoplasmic reticulum vesicles isolated from excitable tissues. Native channels from mammalian or amphibian skeletal muscle displayed three different calcium dependencies, cardiac (C), mammalian skeletal (MS), and low fractional open times (low P-o), as reported for channels from brain cortex. Native channels from cardiac muscle presented only the MS and C dependencies. Channels with the MS or low P-o behaviors showed bell-shaped calcium dependencies, but the latter had fractional open times of <0.1 at all [Ca2+]. Channels with C calcium dependence were activated by[Ca2+] < 10 mu M and were not inhibited by increasing cis [Ca2+] up to 0.5 mM. After oxidation with 2,2'-dithiodipyridine or thimerosal, channels with low P-o or MS dependencies increased their activity. Thesechannels modified their calcium dependencies sequentially, from low P-o to MS and C, or from MS to C. Reduction with glutathione of channels with C dependence (native or oxidized) decreased their fractional open times in 0.5 mM cis [Ca2+], from near unity to 0.1-0.3. These results show that all native channels displayed at least two calcium dependencies regardless of their origin, and that these changed after treatment with redox reagents.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 07:01:51