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Titolo:
COMPARATIVE-STUDY OF CA MG-DEPENDENT NUCL EASES OF THE MAMMALIAN THYMUS/
Autore:
NIKONOVA LV; NANAZASHVILI MG; KROTOVA KE; NAZAROVA LF; RABAYA NA; PRUSAKOVA OV; BELETSKII IP;
Indirizzi:
RUSSIAN ACAD SCI,INST THEORET & EXPT BIOPHYS PUSHCHINO 142292 MOSCOW OBLAST RUSSIA
Titolo Testata:
Izvestia Akademii nauk SSSR. Seria biologiceskaa
fascicolo: 2, , anno: 1998,
pagine: 180 - 186
SICI:
0002-3329(1998):2<180:COCMNE>2.0.ZU;2-D
Fonte:
ISI
Lingua:
RUS
Soggetto:
APOPTOTIC RAT THYMOCYTES; PROGRAMMED CELL-DEATH; DNA-DEGRADATION; LYMPHOID-TISSUES; ENDONUCLEASE; PURIFICATION; CALCIUM; ACTIVATION; CHROMATIN; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
L.V. Nikonova et al., "COMPARATIVE-STUDY OF CA MG-DEPENDENT NUCL EASES OF THE MAMMALIAN THYMUS/", Izvestia Akademii nauk SSSR. Seria biologiceskaa, (2), 1998, pp. 180-186

Abstract

Ca/Mg-dependent nuclease is a possible key enzyme of apoptosis, We isolated and purified nucleases from the human and rat thymus to a homogeneous state and compared some properties of the obtained preparationswith those of the earlier isolated Ca/Mg-dependent nuclease from the calf thymus, The activity of the nuclease from the human thymus in thepresence of bivalent ions decreases in a sequence: (Ca + Mg) = (Ca + Mn) > Mn, that from the rat thymus: (Ca + Mn) > Mn > (Ca + Mg), and that from the calf thymus: (Ca + Mn) > (Ca + Mg) > Mn. Nuclease are not active in a medium containing only Mg, Ca, Co, and Zn ions. The preparations proved to be unstable during their isolation and storage. If the relative molecular mass of the purifies preparations was according to electrophoresis in 12% DS-Na-polyacrylamide gel 28, 29, and 18.4 and21 kDa for the calf, human, and rat, respectively, after storage at -20 degrees C for two to six months, the molecular mass of native proteins decreases to 17-14 kDa. Some other properties of the enzymes have been described.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/05/20 alle ore 01:18:36