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Titolo:
MICE LACKING NEUTROPHIL ELASTASE REVEAL IMPAIRED HOST-DEFENSE AGAINSTGRAM-NEGATIVE BACTERIAL SEPSIS
Autore:
BELAAOUAJ A; MCCARTHY R; BAUMANN M; GAO ZM; LEY TJ; ABRAHAM SN; SHAPIRO SD;
Indirizzi:
WASHINGTON UNIV,SCH MED,BARNES JEWISH HOSP,DEPT CELL BIOL ST LOUIS MO63110 WASHINGTON UNIV,SCH MED,BARNES JEWISH HOSP,DEPT CELL BIOL ST LOUIS MO63110 WASHINGTON UNIV,SCH MED,BARNES JEWISH HOSP,DEPT MED ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,BARNES JEWISH HOSP,DEPT GENET ST LOUIS MO 63110 DUKE UNIV,MED CTR,DEPT PATHOL & MICROBIOL DURHAM NC 27710
Titolo Testata:
Nature medicine
fascicolo: 5, volume: 4, anno: 1998,
pagine: 615 - 618
SICI:
1078-8956(1998)4:5<615:MLNERI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINEAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
A. Belaaouaj et al., "MICE LACKING NEUTROPHIL ELASTASE REVEAL IMPAIRED HOST-DEFENSE AGAINSTGRAM-NEGATIVE BACTERIAL SEPSIS", Nature medicine, 4(5), 1998, pp. 615-618

Abstract

Neutrophil elastase (NE) is a potent serine proteinase(1,2) whose expression is limited to a narrow window during myeloid development. In neutrophils, NE is stored in azurophil granules along with other serineproteinases(3,4) (cathepsin G, proteinase 3 and azurocidin) at concentrations exceeding 5 mM (ref. 5). As a result of its capacity to efficiently degrade extracellular matrix, NE has been implicated in a variety of destructive diseases(6). Indeed, while much interest has focusedon the pathologic effects of this enzyme, little is known regarding its normal physiologic function(s). Because previous in vitro data haveshown that NE exhibits antibacterial activity(7-9), we investigated the role of NE in host defense against bacteria. Generating strains of mice deficient in NE (NE-/-) by targeted mutagenesis, we show that NE-/- mice are more susceptible than their normal littermates to sepsis and death following intraperitoneal infection with Gram negative (Klebsiella pneumoniae and Escherichia coli) but not Cram positive (Staphylococcus aureus) bacteria. Our data indicate that neutrophils migrate normally to sites of infection in the absence of NE, but that NE is required for maximal intracellular killing of Gram negative bacteria by neutrophils.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/05/20 alle ore 15:17:05