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Titolo:
1,25-DIHYDROXY-VITAMIN-D-3 INHIBITS ADIPOCYTE DIFFERENTIATION AND GENE-EXPRESSION IN MURINE BONE-MARROW STROMAL CELL CLONES AND PRIMARY CULTURES
Autore:
KELLY KA; GIMBLE JM;
Indirizzi:
UNIV OKLAHOMA,HLTH SCI CTR,DEPT SURG,WILLIAMS PAVIL 2140,WP2140,920 STANTON L YOUNG BL OKLAHOMA CITY OK 73190 UNIV OKLAHOMA,HLTH SCI CTR,DEPT SURG OKLAHOMA CITY OK 73190 UNIV OKLAHOMA,HLTH SCI CTR,DEPT PATHOL OKLAHOMA CITY OK 73190 UNIV OKLAHOMA,HLTH SCI CTR,DEPT ORTHODONT OKLAHOMA CITY OK 73190 OKLAHOMA MED RES FDN OKLAHOMA CITY OK 73104
Titolo Testata:
Endocrinology
fascicolo: 5, volume: 139, anno: 1998,
pagine: 2622 - 2628
SICI:
0013-7227(1998)139:5<2622:1IADAG>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT CALVARIA CELLS; 3RD COMPONENT; 3T3-L1 CELLS; OSTEOBLASTIC CELLS; LYMPHOCYTE GROWTH; COMPLEMENT C3; 1-ALPHA,25-DIHYDROXYVITAMIN-D3; MOUSE; LINE; ADIPOGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
K.A. Kelly e J.M. Gimble, "1,25-DIHYDROXY-VITAMIN-D-3 INHIBITS ADIPOCYTE DIFFERENTIATION AND GENE-EXPRESSION IN MURINE BONE-MARROW STROMAL CELL CLONES AND PRIMARY CULTURES", Endocrinology, 139(5), 1998, pp. 2622-2628

Abstract

Bone marrow stromal stem cells differentiate into adipocytes and osteoblasts. These two Lineages are thought to be reciprocally related, inpart due to the observation that the osteoblast-inducing factor, 1,25dihydroxy vitamin D-3 [1,25(OH)(2)D-3], inhibited adipogenesis of ratfemoral-derived stromal cell cultures. However, the literature is divided concerning the adipogenic effects of this steroid hormone. This work examined the effect of 1,25(OH)(2)D-3 (10(-12)-10(-8) M) On murinefemoral-derived bone marrow stromal cell differentiation in response to adipogenic agonists employing two different classes of nuclear hormone receptors: the glucocorticoid receptor (hydrocortisone) or peroxisome proliferator-activated receptors (thiazolidinediones). Experimentsused the multipotent murine bone marrow stromal cell line, BMS2, and its subclones, as well as primary-derived murine hone marrow stromal cell cultures. In all systems examined, 1,25(OH)(2)D-3 blocked adipogenesis induced by hydrocortisone, methylisobutylxanthine, and indomethacin based on flow cytometric analysis of lipid accumulation. This correlated with reduced messenger RNA levels of the late adipocyte gene markers, aP2 and adipsin. In the BMS2 subclone no. 24, the 1,25(OH)(2)D-3actions were concentration dependent, Whereas 1,25(OH)(2)D-3 partially inhibited thiazolidinedione-induced adipogenesis in the parental BMS2 cell line, it had minimal effect on the thiazolidinediane-induced differentiation of the BMS2 subclone and primary cultures. These findings indicate that 1,25(OH)(2)D-3, at nanomolar concentrations, completely inhibits murine bone marrow stromal cell differentiation in responseto glucocorticoid-based adipogenic agonists but is a less effective adipogenic antagonist following induction with thiazolidinediones. Thiswork supports the conclusion that 1,25(OH)(2)D-3 inhibits murine femoral-derived bone marrow stromal cell adipogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:02:15