Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EFFECT OF 17-BETA-ESTRADIOL ON SOMATOSTATIN RECEPTOR EXPRESSION AND INHIBITORY EFFECTS ON GROWTH-HORMONE AND PROLACTIN-RELEASE IN RAT PITUITARY CELL-CULTURES
Autore:
DJORDJIJEVIC D; ZHANG J; PRIAM M; VIOLLET C; GOURDJI D; KORDON C; EPELBAUM J;
Indirizzi:
INSERM U159,CTR PAUL BROCA,2TER RUE ALESIA F-75014 PARIS FRANCE INSERM U159,CTR PAUL BROCA F-75014 PARIS FRANCE
Titolo Testata:
Endocrinology
fascicolo: 5, volume: 139, anno: 1998,
pagine: 2272 - 2277
SICI:
0013-7227(1998)139:5<2272:EO1OSR>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RIBONUCLEIC-ACID; ANTERIOR-PITUITARY; ADENYLATE-CYCLASE; PHENOL RED; IDENTIFICATION; SECRETION; SUBTYPES; BINDING; RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
D. Djordjijevic et al., "EFFECT OF 17-BETA-ESTRADIOL ON SOMATOSTATIN RECEPTOR EXPRESSION AND INHIBITORY EFFECTS ON GROWTH-HORMONE AND PROLACTIN-RELEASE IN RAT PITUITARY CELL-CULTURES", Endocrinology, 139(5), 1998, pp. 2272-2277

Abstract

In the present study, we tested whether 17 beta-estradiol (E-2)-induced PRL sensitivity to somatostatin-14 (SRIF) involves selective upregulation of discrete somatostatin receptor subtypes (ssts) in primary cultures of female rat pituitary cells. The efficacy of the endogenous peptide SRIF to inhibit GH and PRL secretion and cAMP accumulation was compared with those of octreotide (OCT), BIM-23052, BIM-23056, and BIM-23268, which have been reported to be relatively selective for rat sst2, sst3, and sst5. Experiments were performed in steroid-depleted media supplemented or not with 1 nM E-2 for 96 h. SRIF, OCT, and BIM-23052 inhibited cAMP accumulation and GH release independently of E-2. In contrast, all three agonists affected PRL release in E-2-treated cultures only. Inhibition of cAMP accumulation by SRIF, OCT, and BIM-23052 was enhanced by exposure of cells to E-2. The rank of potency of the agonists, OCT = SRIF > BIM-23052, was similar for GH and PRL inhibition, BIM-23268 was a weak agonist on GH, but not on PRL, secretion. BIM-23056 had no effect on the release of either hormone, but slightly inhibited cAMP formation in E-2-treated cells. To verify whether SRIF receptor gene expression correlated with these observations, messenger RNA(mRNA) transcripts corresponding to the five ssts were measured by quantitative RT-PCR in the presence or absence of E-2. Control cells expressed predominantly sst2 and ssts transcripts; sst1 mRNA was present in moderate amounts, whereas sst4 and sst5 were only weakly expressed. E-2 had a differential effect on distinct ssts; it increased mRNA concentrations corresponding to sst2 and sst3, and decreased that of sst1. These results indicate that sst2 and ssts receptors are the major somatostatin receptors expressed in the female rat pituitary, and that both of them are positively regulated by estradiol. However, the capacity of lactotropes to respond to SRIF after exposure to E-2 seems to depend more upon E-2-induced upregulation of the sst2 than of the sst3 receptor subtype.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:49:16